Induction of 1-Acylglycerophosphocholine Acyltransferase Genes by Fibrates in the Liver of Rats
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- Yamazaki Tohru
- Faculty of Pharmaceutical Sciences, Josai University
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- Wakabayashi Michiko
- Faculty of Pharmaceutical Sciences, Josai University
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- Ikeda Erika
- Faculty of Pharmaceutical Sciences, Josai University
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- Tanaka Shizuyo
- Faculty of Pharmaceutical Sciences, Josai University
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- Sakamoto Takeshi
- Faculty of Pharmaceutical Sciences, Josai University
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- Mitsumoto Atsushi
- Faculty of Pharmaceutical Sciences, Josai International University
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- Kudo Naomi
- Faculty of Pharmaceutical Sciences, Josai University
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- Kawashima Yoichi
- Faculty of Pharmaceutical Sciences, Josai University
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抄録
The effect of fibrates (clofibric acid, bezafibrate and fenofibrate) on the gene expression and activity of 1-acylglycerophosphocholine acyltransferase (LPCAT) was investigated. The administration of 0.1% (w/w) clofibric acid, bezafibrate or fenofibrate in diet for 14 d to rats induced LPCAT activity in hepatic microsomes in the following order: fenofibrate>bezafibrate>clofibric acid. The LPCAT induced by fenofibrate preferred to arachidonoyl-CoA and linoleoyl-CoA to a greater extent than did LPCAT in control microsomes. The treatment with the fibrates resulted in upregulation of the relative expression of mRNAs encoding LPCAT3 and LPCAT4 in the following order: fenofibrate>bezafibrate>clofibric acid. The administration of fibrates did not change the expression of genes encoding either LPCAT1 or LPCAT2. The treatment with fibrates elevated relative levels of both mRNAs encoding Δ6 desaturase (Fads2) and Δ5 desaturase (Fads1) in the order of fenofibrate>bezafibrate>clofibric acid, and the extent of the increase in the level of Δ6 desaturase mRNA was greater than that of Δ5 desaturase. Fatty acid profile in hepatic phosphatidylcholine (PC) was significantly changed by the treatments with fibrates. These results suggest (i) that fibrates induce LPCAT activity in hepatic microsomes by elevating the expression of genes encoding LPCAT3 and LPCAT4, (ii) that the changes in fatty acid profile of hepatic PC are, in part, due to the elevated expression of two isoforms, LPCAT3 and LPCAT4, and (iii) that the ability of fibrates to induce these changes are in the order of fenofibrate>bezafibrate>clofibric acid.
収録刊行物
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- Biological & Pharmaceutical Bulletin
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Biological & Pharmaceutical Bulletin 35 (9), 1509-1515, 2012
公益社団法人 日本薬学会
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詳細情報 詳細情報について
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- CRID
- 1390282679610802688
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- NII論文ID
- 130001872074
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- NII書誌ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC38bmslamsA%3D%3D
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- ISSN
- 13475215
- 09186158
- http://id.crossref.org/issn/09186158
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- NDL書誌ID
- 023900694
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- PubMed
- 22975502
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可