Molecular and Functional Analysis of Cellular Phenomena Using Single-Cell Mass Spectrometry

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Author(s)

Abstract

Single-cell analysis has attracted attention in many fields of biological studies as a tool to survey the precise mechanisms of cellular and molecular behavior. The development of sensitive mass spectrometry allows the study of molecules in single cells or small regions. Matrix-assisted laser desorption/ionization imaging mass spectrometry and secondary-ion mass spectrometry use <i>in situ</i> ionization of specimens on sample plates to visualize molecular distributions as images from mass spectra. Several single-cell mass spectrometry technologies that initially recover a single cell followed by ionization have been developed. Among them, only nanospray-mediated sampling and ionization named Live Single-cell Mass Spectrometry can be used for real-time analysis. This paper explains that method in detail.

Journal

  • Biological and Pharmaceutical Bulletin

    Biological and Pharmaceutical Bulletin 35(9), 1425-1431, 2012

    The Pharmaceutical Society of Japan

Codes

  • NII Article ID (NAID)
    130001872094
  • NII NACSIS-CAT ID (NCID)
    AA10885497
  • Text Lang
    ENG
  • ISSN
    0918-6158
  • NDL Article ID
    023900324
  • NDL Call No.
    Z53-V41
  • Data Source
    NDL  J-STAGE 
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