Water Extracts of Immature <i>Rubus coreanus</i> Regulate Lipid Metabolism in Liver Cells

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Author(s)

    • Marahatta Anu [他] Chae Soo-Wan
    • Department of Pharmacology, Institute of Cardiovascular Research, School of Medicine, Chonbuk National University|Clinical Trial Center for Functional Foods, Chonbuk National University Hospital
    • Chae Han-Jung
    • Department of Pharmacology, Institute of Cardiovascular Research, School of Medicine, Chonbuk National University
    • Lee Geum-Hwa
    • Department of Dental Pharmacology, Wonkwang Dental Research Institute, Dental School, Wonkwang University
    • Marahatta Anu
    • Department of Pharmacology, Institute of Cardiovascular Research, School of Medicine, Chonbuk National University
    • Lee Hak-Yong
    • Department of Pharmacology, Institute of Cardiovascular Research, School of Medicine, Chonbuk National University
    • Kim Sun-Young
    • Clinical Trial Center for Functional Foods, Chonbuk National University Hospital
    • So Byung-Ok
    • Clinical Trial Center for Functional Foods, Chonbuk National University Hospital

Abstract

Hyperlipidemia is a major contributor for atherosclerosis and hypolipidemic drugs such as statin are highly prescribed to treat elevated lipid level in plasma. <i>Rubus coreanus</i>, which is widely cultivated in south eastern Asia, have been reported to show significant cholesterol lowering action in hyperlipidemic subjects. Our objective was to determine the cellular effect of <i>Rubus coreanus</i> extract (RCE) on cholesterol biosynthesis in human hepatic cells (HepG2) and to elucidate the molecular mechanism by which it causes change in cholesterol metabolism. RCE treatment lowered cholesterol biosynthesis as well as secretion from HepG2 cells. This effect was associated with lowering the release of apolipoproteins from hepatic cells. RCE treatment also showed an increase in phosphorylation of foxhead box protein 01 (FoXo-1) and 5-adenosine monophosphate-activated protein kinase (AMPK), thus lowering expression of phosphoenolpyruvate carboxykinase (PEPCK) and G6Pase, which might be a major pathway for cholesterol biosynthesis inhibition. Apart from this; RCE also lowered sterol regulatory element-binding protein-1 (SREBP-1) expression in HepG2 cells, showing a long term regulation of cholesterol biosynthesis activity. These results indicate that one of the anti-hyperlipidemic actions of RCE is due to inhibition of cholesterol biosynthesis in hepatic cells and provides first documentation of a hypolipidemic bio-molecular action of <i>Rubus coreanus</i>.

Journal

  • Biological and Pharmaceutical Bulletin

    Biological and Pharmaceutical Bulletin 35(11), 1907-1913, 2012

    The Pharmaceutical Society of Japan

Codes

  • NII Article ID (NAID)
    130001872247
  • NII NACSIS-CAT ID (NCID)
    AA10885497
  • Text Lang
    ENG
  • ISSN
    0918-6158
  • NDL Article ID
    024032706
  • NDL Call No.
    Z53-V41
  • Data Source
    NDL  J-STAGE 
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