Gomisin A Enhances Tumor Necrosis Factor-α-Induced G1 Cell Cycle Arrest <i>via</i> Signal Transducer and Activator of Transcription 1-Mediated Phosphorylation of Retinoblastoma Protein
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- Waiwut Pornthip
- Division of Pathogenic Biochemistry, Institute of Natural Medicine, University of Toyama
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- Shin Myoung-Sook
- Division of Pathogenic Biochemistry, Institute of Natural Medicine, University of Toyama
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- Yokoyama Satoru
- Division of Pathogenic Biochemistry, Institute of Natural Medicine, University of Toyama
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- Saiki Ikuo
- Division of Pathogenic Biochemistry, Institute of Natural Medicine, University of Toyama
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- Sakurai Hiroaki
- Division of Pathogenic Biochemistry, Institute of Natural Medicine, University of Toyama Department of Cancer Cell Biology, Graduate School of Medicine and Pharmaceutical Sciences, University of Toyama
Bibliographic Information
- Other Title
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- Gomisin A Enhances Tumor Necrosis Factor-α-Induced G1 Cell Cycle Arrest via Signal Transducer and Activator of Transcription 1-Mediated Phosphorylation of Retinoblastoma Protein
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Abstract
Gomisin A, a dibenzocyclooctadiene lignan isolated from the fruit of Schisandra chinensis, has been reported as an anti-cancer substance. In this study, we investigated the effects of gomisin A on cancer cell proliferation and cell cycle arrest in HeLa cells. Gomisin A significantly inhibited cell proliferation in a dose-dependent manner after 72 h treatment, especially in the presence of tumor necrosis factor-α (TNF-α), due to cell cycle arrest in the G1 phase with the downregulation of cyclin D1 expression and Retinoblastoma (RB) phosphorylation. In addition, gomisin A in combination with TNF-α strongly suppressed the expression of signal transducer and activator of transcription 1 (STAT1). Inhibition of STAT1 pathways by a small-interfering RNA against STAT1 and AG490 Janus kinase (JAK) kinase inhibitor AG490 reduced the cyclin D1 expression and RB phosphorylation, indicating that JAK-mediated STAT1 activation is involved in gomisin A-induced G1 cell cycle arrest.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 35 (11), 1997-2003, 2012
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390001204633470336
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- NII Article ID
- 130001872260
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- NII Book ID
- AA10885497
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- COI
- 1:STN:280:DC%2BC3s7gs1WqsQ%3D%3D
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 024032964
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- PubMed
- 23123471
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed