The Use of an Artificial Skin Model to Study Transdermal Absorption of Drugs in Inflamed Skin
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Studies on drug disposition in inflamed skin are important for safe and effective application of topical drugs. Here, the absorption of flurbiprofen (FP) through inflamed skin was examined <i>in vivo</i> and in a skin-mimicking artificial model system. The model skin system consisted of a silicone membrane acting as a model stratum corneum, laminated dialysis membranes acting as a model of viable skin, and 2 microdialysis probes—one used for determination of FP concentration and one acting as a model vessel. This model system could be used for quantitative evaluation of complicated permeation processes. In the <i>in vivo</i> experiments, FP absorption was suppressed in rats with inflamed skin induced by an intracutaneous injection of a mixed solution of λ-carrageenan, zymosan, and casein. Bovine serum albumin solution was placed between the dialysis membranes in the model skin system to mimic protein leaching in skin; the results suggested that the delayed absorption of FP in inflamed skin was due to binding to serum proteins leaching in the tissue. Such a combination of <i>in vivo</i> experiments and a model skin system is useful for understanding complex phenomena in inflamed and damaged skin and reduces experimental animal use.
- Biological and Pharmaceutical Bulletin
Biological and Pharmaceutical Bulletin 35(2), 203-209, 2012
The Pharmaceutical Society of Japan