Pharmacodynamic Characterization of Nitric Oxide-Mediated Vasodilatory Activity in Isolated Perfused Rat Mesenteric Artery Bed
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- Inoue Shinsuke
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Aiba Tetsuya
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Masaoka Yasuyuki
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Shimizu Keiko
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Komori Yukiko
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Mio Mitsunobu
- School of Pharmacy, Shujitsu University
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- Takatori Shingo
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Kawasaki Hiromu
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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- Kurosaki Yuji
- Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama University
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Abstract
Vasodilation profiles following a short-term infusion of nitric oxide (NO), acetylcholine (ACh), and sodium nitroprusside (SNP) into an isolated perfused mesenteric artery bed were analyzed in rats to examine their vasodilatory efficacy under physiological conditions. These compounds commonly increase the intracellular NO concentration to exert vasodilatory activity. In an experiment with exogenous NO infusion where 100 μl of 1 : 300 diluted NO-saturated solution (approx. 53 pmol of NO) was applied, the infusion caused transient vasodilation in a dose-dependent manner, with the peak vasodilation value being 74.7% of the maximum relaxation value. In experiments with ACh, the peak vasodilation value was 81.5% of the maximum at a dose of 60 pmol. The vasodilation profile of ACh was similar to that of NO infusion, but the ACh-induced vasodilation reduced at a slower rate than that induced by NO infusion. The vasodilatory activity of SNP was less potent than that of ACh, and its peak value was 62.8% of the maximum at a dose of 2000 pmol. However, SNP activity was augmented by removing the vascular endothelia of the mesenteric artery bed, and the peak value reached 67.3% of the maximum at a dose of 60 pmol. Pharmacodynamic analysis indicated that NO and ACh are equivalent regarding their vasodilatory efficacy, while the efficacy of SNP was less than 1% of theirs, as the arterial vascular endothelium impeded intracellular SNP-related NO generation, by which 95% of SNP's vasodilatory efficacy was negated. These findings will be helpful to understand factors influencing the therapeutic efficacy of vasodilators.
Journal
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- Biological and Pharmaceutical Bulletin
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Biological and Pharmaceutical Bulletin 34 (9), 1487-1492, 2011
The Pharmaceutical Society of Japan
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Details 詳細情報について
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- CRID
- 1390282679608168960
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- NII Article ID
- 130001872445
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- NII Book ID
- AA10885497
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- ISSN
- 13475215
- 09186158
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- NDL BIB ID
- 11217498
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- PubMed
- 21881238
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed