Expression of <i>p53</i>-Regulated Proteins in Human Cultured Lymphoblastoid TSCE5 and WTK1 Cell Lines during Spaceflight

• TAKAHASHI Akihisa

  Advanced Scientific Research Leaders Development Unit, Gunma University Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency

• SUZUKI Hiromi

  Japan Space Forum Space Environmental Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University

• OMORI Katsunori

  Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency

• SEKI Masaya

  Space Environmental Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University Advanced Engineering Services Co., Ltd.

• HASHIZUME Toko

  Space Environmental Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University Advanced Engineering Services Co., Ltd.

• SHIMAZU Toru

  Japan Space Forum

• ISHIOKA Noriaki

  Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency Space Environmental Medicine, Graduate School of Medical and Dental Sciences, Kagoshima University

• OHNISHI Takeo

  Institute of Space and Astronautical Science, Japan Aerospace Exploration Agency Department of Radiation Oncology, School of Medicine, Nara Medical University

Bibliographic Information

Other Title

• Expression of p53-Regulated Proteins in Human Cultured Lymphoblastoid TSCE5 and WTK1 Cell Lines during Spaceflight

Abstract

The aim of this study was to determine the biological effects of space radiations, microgravity, and the interaction of them on the expression of p53-regulated proteins. Space experiments were performed with two human cultured lymphoblastoid cell lines: one line (TSCE5) bears a wild-type p53 gene status, and another line (WTK1) bears a mutated p53 gene status. Under 1 gravity or microgravity conditions, the cells were grown in the cell biology experimental facility (CBEF) of the International Space Station for 8 days without experiencing the stress during launching and landing because the cells were frozen during these periods. Ground control samples were simultaneously cultured for 8 days in the CBEF on the ground for 8 days. After spaceflight, protein expression was analyzed using a Panorama^TM Ab MicroArray protein chips. It was found that p53-dependent up-regulated proteins in response to space radiations and space environment were MeCP2 (methyl CpG binding protein 2), and Notch1 (Notch homolog 1), respectively. On the other hand, p53-dependent down-regulated proteins were TGF-β, TWEAKR (tumor necrosis factor-like weak inducer of apoptosis receptor), phosho-Pyk2 (Proline-rich tyrosine kinase 2), and 14-3-3θ/τ which were affected by microgravity, and DR4 (death receptor 4), PRMT1 (protein arginine methyltransferase 1) and ROCK-2 (Rho-associated, coiled-coil containing protein kinase 2) in response to space radiations. ROCK-2 was also suppressed in response to the space environment. The data provides the p53-dependent regulated proteins by exposure to space radiations and/or microgravity during spaceflight. Our expression data revealed proteins that might help to advance the basic space radiation biology.

Journal

• Journal of Radiation Research

  Journal of Radiation Research 53 (2), 168-175, 2012

  Journal of Radiation Research Editorial Committee

Keywords

• Space radiations
• Microgravity
• p53
• Protein expression
• Spaceflight

Details

• CRID: 1390282680192318848
• NII Article ID: 130001876839
• NII Book ID: AA00705792
• DOI: 10.1269/jrr.11140
• COI: 1:STN:280:DC%2BC38vks12gsQ%3D%3D
• ISSN: 13499157
• ISSN: 04493060
• NDL BIB ID: 023572074
• PubMed: 22374402
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023572074
• Web Site: http://academic.oup.com/jrr/article-pdf/53/2/168/2710508/jrr-53-168.pdf
• Web Site: https://search.jamas.or.jp/link/ui/2013012581
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed