A PCR Based Survey of <i>Babesia ovata</i> in Cattle from Various Asian, African and South American Countries

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  • YOSHINARI Takeshi
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
  • SIVAKUMAR Thillaiampalam
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
  • ASADA Masahito
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
  • BATTSETSEG Badgar
    Laboratory of Molecular Genetics, Institute of Veterinary Medicine, Ulaanbaatar, Mongolia
  • HUANG Xiaohong
    Fujian Key Laboratory of Developmental Biology and Neuroscience, College of Life Science, Fujian Normal University, Fujian, China
  • LAN Dinh Thi Bich
    Institute of Resources Environment and Biotechnology, Hue University, Hue, Vietnam
  • INPANKAEW Tawin
    Department of Parasitology, Faculty Medicine, Kasetsart University, Bangkok, Thailand
  • YBAÑEZ Adrian P.
    Department of Veterinary Clinical Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
  • ALHASSAN Andy
    Veterinary Services Laboratory, Accra, Ghana
  • THEKISOE Oriel M. M.
    Parasitology Research Program, Department of Zoology and Entomology, University of the Free State Qwaqwa Campus, Phuthaditjhaba, South Africa
  • DE MACEDO Alan Caine Costa
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
  • INOKUMA Hisashi
    Department of Veterinary Clinical Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
  • IGARASHI Ikuo
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan
  • YOKOYAMA Naoaki
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080–8555, Japan

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  • A PCR Based Survey of Babesia ovata in Cattle from Various Asian, African and South American Countries

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Abstract

Babesia ovata is a tick-transmitted hemoprotozoan parasite that infects cattle. In our study, bovine blood samples (n=2,034) were collected from 10 different countries (Brazil, China, Ghana, Japan, Mongolia, the Philippines, South Africa, Sri Lanka, Thailand and Vietnam) and DNA extracted. The DNA samples were screened using an established and specific polymerase chain reaction (PCR) assay targeting the Apical membrane antigen 1 (AMA-1) gene. Parasite DNA was detected among samples collected from Japan, Mongolia and Thailand. Sequence analyses confirmed that the PCR assay detected only B. ovata AMA-1, and that amplicons from different geographical locations were conserved. Our findings highlight the importance of designing adequate strategies to control B. ovata infection in Japan, Mongolia, and Thailand.

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