PCR-Based Detection of <i>Leishmania donovani</i> DNA in a Stray Dog from a Visceral Leishmaniasis Endemic Focus in Bangladesh

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Author(s)

    • ALAM Mohammad Zahangir ALAM Mohammad Zahangir
    • Department of Parasitology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh–2202, Bangladesh|Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-ku, Sapporo 060–0818, Japan
    • KATO Hirotomo [他] KATO Hirotomo
    • Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-ku, Sapporo 060–0818, Japan
    • SAKURAI Tatsuya
    • Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-ku, Sapporo 060–0818, Japan
    • KATAKURA Ken
    • Laboratory of Parasitology, Department of Disease Control, Graduate School of Veterinary Medicine, Hokkaido University, Kita 18 Nishi 9, Kita-ku, Sapporo 060–0818, Japan

Abstract

Although <i>Phlebotomus argentipes</i> as the only known vector of visceral leishmaniasis (VL) is zoophilic in nature, VL is considered to be anthroponotic in the Indian subcontinent. Peripheral blood samples from 85 stray dogs were examined for any molecular evidence of <i>Leishmania</i> infection in VL endemic areas of Bangladesh. Parasite DNA was detected in a blood sample from 1 of 85 (1.2%) stray dogs using ITS1-PCR, and PCR sequencing of the rRNA-ITS and cytochrome <i>b</i> gene confirmed that the parasitic DNA was <i>Leishmania donovani</i>. The results support the assumption that dogs are a probable animal reservoir for the <i>Leishmania</i> parasite in Bangladesh. It will be important to investigate the possible epidemiological role of dogs in domestic foci of VL endemic areas in Bangladesh.

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 75(1), 75-78, 2013

    JAPANESE SOCIETY OF VETERINARY SCIENCE

Codes

  • NII Article ID (NAID)
    130001879680
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • ISSN
    0916-7250
  • NDL Article ID
    024246196
  • NDL Call No.
    Z18-350
  • Data Source
    NDL  J-STAGE 
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