Detection of <i>Nesopora caninum</i>-Specific DNA from Cerebrospinal Fluid by Polymerase Chain Reaction in a Dog with Confirmed Neosporosis

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  • ISHIGAKI Kyohei
    Noya Animal Hospital Noya Animal Hospital, 143–19 Kamikayama, Hidaka, Saitama 350–1234, Japan
  • NOYA Masahiko
    Noya Animal Hospital Noya Animal Hospital, 143–19 Kamikayama, Hidaka, Saitama 350–1234, Japan
  • KAGAWA Yumiko
    North Lab North Lab, 8–35 Kita, 2 Hondori, Shiroishi, Sapporo, Hokkaido 003–0027, Japan
  • IKE Kazunori
    Laboratory of Veterinary Parasitology, Department of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University Laboratory of Veterinary Parasitology, Department of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1–7–1 Kyonan, Musashino, Tokyo 180–8602, Japan
  • ORIMA Hiromitsu
    Laboratory of Veterinary Radiology, Department of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University Laboratory of Veterinary Radiology, Department of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1–7–1 Kyonan, Musashino, Tokyo 180–8602, Japan
  • IMAI Soichi
    Laboratory of Veterinary Parasitology, Department of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University Laboratory of Veterinary Parasitology, Department of Veterinary Medicine, Faculty of Veterinary Science, Nippon Veterinary and Life Science University, 1–7–1 Kyonan, Musashino, Tokyo 180–8602, Japan

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  • Detection of Nesopora caninum-Spcific DNA from Cerebrospinal Fluid by Polymerase Chain Reaction in a Dog with Confirmed Neosporosis

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Abstract

A one-month male Greyhound dog presented with a swinging gait of the hindlimbs, and later developed muscular atrophy of the femoral region and hyperextension of hindlimbs. The dog had positive serum IFAT titers to Neospora caninum, but a negative titer in the cerebrospinal fluid (CSF). N. caninum-specific DNA was amplified from the CSF using a semi-nested polymerase chain reaction assay. Clusters of protozoa in biopsied muscle fibers were subsequently confirmed as N. caninum tachyzoites by immunohistochemical examination. Early recognition and treatment are necessary for effective recovery of clinical canine neosporosis, but antemortem diagnosis is difficult. We suggest that the detection of parasite deoxyribonucleic acid in the CSF is a useful antemortem diagnostic method in facilitating treatment of this disease.

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