Sensitive Detection of Whole-Genome Differentiation among Closely-Related Species of the Genus <i>Fusarium</i> Using DNA-DNA Hybridization and a Microplate Technique

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  • WATANABE Maiko
    Division of Microbiology, National Institute of Health Sciences Division of Microbiology, National Institute of Health Sciences, 1–18–1 Kamiyoga, Setagaya-ku, Tokyo 158–8501, Japan
  • GOTO Keiichi
    Food Research Laboratories, Mitsui Norin Co., Ltd. Food Research Laboratories, Mitsui Norin Co., Ltd., 223–1 Miyabara, Fujieda, Shizuoka 426–0133, Japan
  • SUGITA-KONISHI Yoshiko
    Division of Microbiology, National Institute of Health Sciences Division of Microbiology, National Institute of Health Sciences, 1–18–1 Kamiyoga, Setagaya-ku, Tokyo 158–8501, Japan
  • KAMATA Yoichi
    Division of Microbiology, National Institute of Health Sciences Division of Microbiology, National Institute of Health Sciences, 1–18–1 Kamiyoga, Setagaya-ku, Tokyo 158–8501, Japan
  • HARA-KUDO Yukiko
    Division of Microbiology, National Institute of Health Sciences Division of Microbiology, National Institute of Health Sciences, 1–18–1 Kamiyoga, Setagaya-ku, Tokyo 158–8501, Japan

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  • Public Health : Sensitive Detection of Whole-Genome Differentiation among Closely-Related Species of the Genus Fusarium Using DNA-DNA Hybridization and a Microplate Technique

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Abstract

We developed a new system for detection of whole-genome differentiation using DNA-DNA hybridization, and tested its sensitivity with three closely-related Fusarium species. We compared DNA-DNA relatedness to nucleotide sequence homologies of five genetic regions between each of five strains of three Fusarium species. DNA-DNA relatedness by our system was 16.2–86.6%. Sequence homologies of 18S rDNA, rDNA cluster region from ITS1 to 28S rDNA, β-tub, EF-1α and lys2 were 100.0, 99.0–100.0, 96.7–100.0, 95.1–99.4, and 94.7–100.0%, respectively. Our system could clearly detect differentiation between closely-related fungal species which have very similar morphological-characteristics, and exhibit little diagnoses in nucleotide sequences. Our results suggest that this system is a good tool for identification and phylogenetic analysis of closely-related fungal species.

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