<i>In Vivo</i> Electroporation Induces Cell Cycle Reentry of Myonuclei in Rat Skeletal Muscle

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Author(s)

    • MIYOSHI Takahiro MIYOSHI Takahiro
    • Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo|Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
    • NAKANO Shin-ichi NAKANO Shin-ichi
    • Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo|Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
    • NAKAMURA Katsuyuki [他] NAKAMURA Katsuyuki
    • Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo|Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
    • YAMANOUCHI Keitaro
    • Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo|Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan
    • NISHIHARA Masugi
    • Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo|Department of Veterinary Physiology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1–1–1 Yayoi, Bunkyo-ku, Tokyo 113–8657, Japan

Abstract

Adult urodele amphibians such as newts are capable of regenerating lost structures including their limbs. In these species, dedifferentiation of myofiber is essential for the regenerative process. Upon terminal differentiation, nuclei of myofiber (myonuclei) are withdrawn from cell cycle, but prior to dedifferentiation, myonuclei reenter the cell cycle. In contrast with urodele amphibians, it is generally accepted that mammalian myofibers are not able to dedifferentiate in response to muscle injury. A recent study has suggested that electroporation can induce dedifferentiation response of skeletal muscle in newt limbs. In the present study, we examined whether myonuclei of skeletal muscle of mammals are capable of reentering the cell cycle by means of electroporation. Electroporation was applied to tibialis anterior muscle of the rat with or without plasmid DNA. Histological analyses revealed that, while electroporation induces degenerative/regenerative responses in skeletal muscle irrespective of the presence of plasmid DNA, the expression of proliferating cell nuclear antigen (PCNA) in myonuclei was observed only in the presence of plasmid DNA. The present results indicate that myonuclei of skeletal muscle are capable of reentering the cell cycle and suggest that <i>in vivo</i> electroporation can induce dedifferentiation of mammalian skeletal muscle.

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 74(10), 1291-1297, 2012

    JAPANESE SOCIETY OF VETERINARY SCIENCE

Codes

  • NII Article ID (NAID)
    130001879837
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • ISSN
    0916-7250
  • NDL Article ID
    024055457
  • NDL Call No.
    Z18-350
  • Data Source
    NDL  J-STAGE 
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