Immunohistochemical Expressions of Main PGE₂ Biosynthesis-related Enzymes and PGE₂ Receptor in Rat Nephrogenesis

• Yamamoto Emi

  Laboratory of Veterinary Pathology, Life and Environmental Sciences, Osaka Prefecture University, Rinkuu Ourai Kita 1-58, Izumisano, Osaka 598-8531, Japan

• Izawa Takeshi

  Laboratory of Veterinary Pathology, Life and Environmental Sciences, Osaka Prefecture University, Rinkuu Ourai Kita 1-58, Izumisano, Osaka 598-8531, Japan

• Kuwamura Mitsuru

  Laboratory of Veterinary Pathology, Life and Environmental Sciences, Osaka Prefecture University, Rinkuu Ourai Kita 1-58, Izumisano, Osaka 598-8531, Japan

• Yamate Jyoji

  Laboratory of Veterinary Pathology, Life and Environmental Sciences, Osaka Prefecture University, Rinkuu Ourai Kita 1-58, Izumisano, Osaka 598-8531, Japan

Bibliographic Information

Other Title

• Immunohistochemical Expressions of Main PGE₂ Biosynthesis-related Enzymes and PGE₂ Receptor in Rat Nephrogenesis

Abstract

Endogenous prostaglandin (PG) E₂ plays important roles in renal homeostasis. Immunoexpressions of PGE₂ biosynthesis-related enzymes, cyclooxygenase (COX)-2 and microsomal PGE₂ synthetase (mPGES)-1 and EP4 (a PGE₂ receptor), were investigated in renal development. Kidney tissues were obtained from fetuses on gestation days 18 and 21 and neonates on days 1 to 18. In fetuses and early neonates, the expressions of COX-2, mPGES-1 and EP4 were observed in developing renal tubules, indicating that COX-2 and its product, PGE₂, play important roles in blastemal cell-derived renal tubular development via EP4. Cyclin D1 expression was seen in both the nucleus and cytoplasm of the developing tubules. These findings differed from the decreased COX-2 expression and exclusive nuclear expression of cyclin D1 seen in abnormal epithelial regeneration of injured renal tubules in cisplatin-treated rats in our previous articles. Collectively, PGE₂, induced by COX-2, regulates renal tubular epithelial formation via EP4.

Journal

• Journal of Toxicologic Pathology

  Journal of Toxicologic Pathology 24 (4), 257-261, 2011

  JAPANESE SOCIETY OF TOXICOLOGIC PATHOLOGY

Keywords

• rat
• nephrogenesis
• PGE₂
• COX-2
• EP4

Details

• CRID: 1390282679392672896
• NII Article ID: 130001884731
• NII Book ID: AN10232280
• DOI: 10.1293/tox.24.257
• ISSN: 1881915X
• ISSN: 09149198
• NDL BIB ID: 023460168
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023460168
• Web Site: https://www.jstage.jst.go.jp/article/tox/24/4/24_4_257/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2012355586
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed