mRNA expression and protein production of the IL-6 cytokine family in human temporomandibular joint synoviocytes stimulated with IL-1<i>β</i> and/or TNF-<i>α</i>

DOI 26 References
  • KAWASHIMA Mutsumi
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo
  • OGURA Naomi
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
  • AKUTSU Miwa
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
  • ITO Ko
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo
  • KONDOH Toshirou
    Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo

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  • IL-1<i>β</i>およびTNF-<i>α</i>刺激ヒト顎関節滑膜細胞におけるIL-6 cytokine familyの発現

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Abstract

Internal derangement (ID) and osteoarthritis (OA) are often encountered in temporomandibular joint (TMJ) disorders. It is known that high concentrations of interleukin (IL)-1β and tumor necrosis factor (TNF)-α are detected in the synovial fluids of patients with ID and OA. IL-1β and TNF-α play key roles in intracapsular pathological conditions of the TMJ. Gene expressions induced by IL-1β and/or TNF-α in synoviocytes obtained from human TMJ were analyzed using microarrays. Microarray analysis showed that levels of IL-6 and leukemia inhibitory factor (LIF), which are in the IL-6 cytokine family, were up-regulated in synoviocytes stimulated with IL-1β and/or TNF-α. The IL-6 cytokine family, which contains IL-6, LIF, oncostatin M (OSM), IL-11, and IL-12, is associated with inflammation and tissue destruction. We examined the gene expressions of IL-6 cytokine family members and their receptors in synoviocytes stimulated with IL-1β and/or TNF-α using RT-PCR, and real-time PCR. The up-regulated levels of IL-6 and LIF were greatest following stimulation with both IL-1β and TNF-α and least following stimulation with only TNF-α. Protein production of IL-6 and LIF also increased in synoviocytes stimulated with IL-1β and/or TNF-α. The up-regulated levels of IL-6 and LIF protein productions reflected the gene expression levels. The gene expressions of OSM, IL-11, and IL-12 were not detected in synoviocytes. Glycoprotein 130 (gp130), which is a receptor subunit for signal transduction of the IL-6 cytokine family, LIF receptor, OSM receptor, and IL-11 receptor were expressed in synoviocytes constitutively. In conclusion, IL-1β and TNF-α may cause abnormalities associated with intracapsular pathological conditions of the TMJ through the enhanced expression of IL-6 and LIF in synoviocytes.<br>

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