Relationship between stx Genotype and Stx2 Expression Level in Shiga Toxin-Producing Escherichia coli O157 Strains

• Kawano Kimiko

  Miyazaki Prefectural Institute for Public Health and Environment

• Ono Hidetoshi

  Hyuga Meat Inspection Center

• Iwashita Osamu

  Tsuno Meat Inspection Center

• Kurogi Mai

  Tsuno Meat Inspection Center

• Haga Takeshi

  Department of Microbiology, University of Miyazaki

• Maeda Ken

  Department of Microbiology, Yamaguchi University

• Goto Yoshitaka

  Department of Microbiology, University of Miyazaki

書誌事項

タイトル別名

• Relationship between <i>stx</i> Genotype and Stx2 Expression Level in Shiga Toxin-Producing <i>Escherichia coli</i> O157 Strains

抄録

To determine the expression level of Shiga toxin (Stx) 2-related toxins (Stx2 and Stx2c) produced by each of 33 Stx-producing Escherichia coli (STEC) O157 strains, stx2 and stx2c mRNAs (stx2-related mRNA) were measured using real-time PCR with primers that recognize sequences common to stx2 and stx2c. The amount of Stx2 and Stx2c protein was measured using a reversed passive latex agglutination (RPLA) kit. Expression of stx2-related mRNA was significantly higher in STEC O157 strains carrying the stx2 gene (i.e., stx2, stx1/stx2, or stx2/stx2c) than in most strains that carried the stx2c gene but not the stx2 gene (i.e., stx2c or stx1/stx2c). RPLA might not measure the precise amount of each toxin variant; nevertheless, stx2-inclusive strains had 40-fold higher mean toxin titers than did strains that carried the stx2c gene but not the stx2 gene, with the exception of 1 stx2c strain. Interestingly, 1 stx2c strain that was isolated from a patient with severe hemorrhagic diarrhea had the highest stx2-related mRNA expression and the highest toxin titer of all 33 STEC O157 strains. Taken together, these findings indicated that measurement of stx2-related mRNA expression could reflect differences in production levels of toxins among STEC strains.

収録刊行物

• Japanese Journal of Infectious Diseases

  Japanese Journal of Infectious Diseases 65 (4), 322-325, 2012

  国立感染症研究所 Japanese Journal of Infectious Diseases 編集委員会

キーワード

• Infectious Diseases
• Microbiology (medical)
• General Medicine

詳細情報

• CRID: 1390282681217995136
• NII論文ID: 130001931831
• NII書誌ID: AA1132885X
• DOI: 10.7883/yoken.65.322
• ISSN: 18842836
• ISSN: 13446304
• NDL書誌ID: 023840639
• PubMed: 22814156
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I023840639
• Web Site: https://search.jamas.or.jp/link/ui/2013338474
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可