Pathological Examination of Lung Tissues in Influenza A Virus-Infected Mice

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Author(s)

    • Fujikura Daisuke
    • Division of Infection and Immunity, Research Center for Zoonosis Control, Hokkaido University
    • Miyazaki Tadaaki
    • Institute for Genetic Medicine, Hokkaido University|Global COE Program, Hokkaido University, Research Center for Zoonosis Control
    • Kimura Takashi
    • Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University
    • Sawa Hirofumi
    • Division of Molecular Pathobiology, Research Center for Zoonosis Control, Hokkaido University|Global COE Program, Hokkaido University, Research Center for Zoonosis Control

Abstract

This study examined pathological changes in the lung tissues of young and aged mice infected with influenza virus. Young mice inoculated with influenza virus showed body weight loss at 4 days post-infection (dpi), meanwhile body weight decrease started from 9 dpi in the aged mice. We histopathologically examined the lungs of these mice. Immunohistochemical examination revealed that viral antigen-positive bronchiolar and alveolar epithelial cell numbers at 3 dpi were significantly higher in young mice than in the aged ones. Further, viral antigen-positive cells were observed at 9 dpi in the aged mice, but not in the young ones. Diffuse and severe bronchointerstitial pneumonia characterized by the accumulation of polymorphonuclear leukocytes (PMNs) was observed in young mice at 6 dpi. Histopathological changes in the aged mice were milder than those in the young mice. Moreover, T cell and macrophage accumulation in the lungs was significantly higher in the young mice than in the aged mice at 9 dpi. These results suggest that there may be a correlation between the relatively low level of infiltration of PMNs, macrophages, and T lymphocytes and the delayed body weight loss and longer lasting infections observed in the lungs of the aged mice. These findings provide detailed insights into the age-specific course of infection in young and aged populations with associated differences in lung pathology.

Journal

  • Japanese Journal of Infectious Diseases

    Japanese Journal of Infectious Diseases 65(5), 383-391, 2012

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

Codes

  • NII Article ID (NAID)
    130001931846
  • NII NACSIS-CAT ID (NCID)
    AA1132885X
  • Text Lang
    ENG
  • ISSN
    1344-6304
  • NDL Article ID
    023971497
  • NDL Call No.
    Z53-C450
  • Data Source
    NDL  J-STAGE 
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