Quantification of Periodontopathic Bacteria in Saliva Using the Invader Assay
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- Tada Akio
- Department of Health Science, Hyogo University
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- Takeuchi Hiroaki
- Department of Translational Research, Tsurumi University, School of Dental Medicine
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- Shimizu Hajime
- Clinical Genomics Development, BML, Inc.
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- Tadokoro Kenichi
- Clinical Genomics Development, BML, Inc.
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- Tanaka Kazuya
- Clinical Genomics Development, BML, Inc.
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- Kawamura Katsumi
- Clinical Genomics Development, BML, Inc.
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- Yamaguchi Toshikazu
- Clinical Genomics Development, BML, Inc.
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- Egashira Toru
- Clinical Genomics Development, BML, Inc.
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- Nomura Yoshiaki
- Department of Translational Research, Tsurumi University, School of Dental Medicine
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- Hanada Nobuhiro
- Department of Translational Research, Tsurumi University, School of Dental Medicine
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Abstract
When quantifying periodontopathic bacteria, it is important to use a convenient method that does not produce false negative results. The Invader assay is a convenient method because it does not involve gene amplification. The purpose of this study was to evaluate the validity of the Invader assay to quantify periodontopathic bacteria. The Invader technology was applied in quantifying five periodontopathic bacteria (Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, Tannerella forsythia, Prevotella intermedia, and Treponema denticola). The Invader assay produced a linear quantitative detection range over concentrations spanning seven exponential values, with a detection limit of 103.7 copies/tube and intra-day and inter-day variance of 0.1% to 4.7% and 0.1% to 3.4%, respectively, in quantifying five periodontopathic bacteria. We compared the results of the Invader assay with those of real-time polymerase chain reaction (PCR) performed for quantifying five periodontopathic bacteria in 22 patients with periodontitis. Among the Invader-detectable bacterial strains of each species, significant correlations were observed in the counts of concerned bacterial species between these two methods, with correlation coefficients ranging from 0.757 to 0.996. This study validated repeatability and reproducibility of the Invader assay in quantifying periodontopathic bacteria and demonstrated consistent agreement between the Invader assay and real-time PCR in quantifying periodontopathic bacteria.
Journal
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- Japanese Journal of Infectious Diseases
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Japanese Journal of Infectious Diseases 65 (5), 415-423, 2012
National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
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Details 詳細情報について
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- CRID
- 1390001206241278976
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- NII Article ID
- 130001931851
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- NII Book ID
- AA1132885X
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- ISSN
- 18842836
- 13446304
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- NDL BIB ID
- 023971613
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- PubMed
- 22996215
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed