拡散測定による細胞内生体高分子の動態解析(I)  [in Japanese] Analysis of bio-macromolecular dynamics (I)  [in Japanese]

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Author(s)

    • 齊藤 健太 Saito Kenta
    • 北海道大学 電子科学研究所 超分子分光研究分野 Research Institute for Electronic Science, Hokkaido University
    • 金城 政孝 Kinjo Masataka
    • 北海道大学 電子科学研究所 超分子分光研究分野 Research Institute for Electronic Science, Hokkaido University

Abstract

Fluorescence correlation spectroscopy (FCS) is a method of measuring molecular diffusion constant and absolute number of molecule at extremely low concentrations based on single-molecule fluorescence detection technique. FCS has proven to be a powerful tool for detection and investigation of bio-molecules in solution. In recent, researchers have applied FCS to study molecular kinetics and biochemical reaction in living cells because FCS can measure the diffusion of molecules in cytosol, plasma membrane region and nucleus distinctly in a living cell. This article provides a short overview of recent FCS study in the field of molecular biology and cell biology. An introduction of FCS principle is provided in the section 2. Recent FCS study is reviewed in the section 3.

Journal

  • Journal of Japanese Society of Biorheology

    Journal of Japanese Society of Biorheology 18(1), 37-43, 2004

    JAPANESE SOCIETY OF BIORHEOLOGY

Codes

  • NII Article ID (NAID)
    130001932197
  • NII NACSIS-CAT ID (NCID)
    AN10042423
  • Text Lang
    JPN
  • Article Type
    特集
  • ISSN
    0913-4778
  • NDL Article ID
    6945022
  • NDL Source Classification
    ZR2(科学技術--生物学--生化学)
  • NDL Call No.
    Z18-1996
  • Data Source
    NDL  J-STAGE 
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