拡散測定による細胞内生体高分子の動態解析(I)  [in Japanese] Analysis of bio-macromolecular dynamics (I)  [in Japanese]

Fluorescence correlation spectroscopy (FCS) is a method of measuring molecular diffusion constant and absolute number of molecule at extremely low concentrations based on single-molecule fluorescence detection technique. FCS has proven to be a powerful tool for detection and investigation of bio-molecules in solution. In recent, researchers have applied FCS to study molecular kinetics and biochemical reaction in living cells because FCS can measure the diffusion of molecules in cytosol, plasma membrane region and nucleus distinctly in a living cell. This article provides a short overview of recent FCS study in the field of molecular biology and cell biology. An introduction of FCS principle is provided in the section 2. Recent FCS study is reviewed in the section 3.