ラクトビオン酸発酵生産条件の検討

• 村上 洋

  大阪市立工業研究所生物化学課

• 瀬古 亜紀子

  竹原化学工業株式会社

• 安積 真澄

  竹原化学工業株式会社

• 上嶋 夏子

  近畿大学農学部

• 吉栖 肇

  近畿大学農学部

• 中野 博文

  大阪市立工業研究所生物化学課

• 北畑 寿美雄

  大阪市立工業研究所生物化学課

書誌事項

タイトル別名

• Fermentative Production of Lactobionic Acid by Burkholderia cepacia

抄録

Lactobionic acid (LA) is derived from lactose (Lac) and has been reported to have several useful functions. LA has been supplied only as a reagent for study because it has been produced chemically in a small scale in Japan. We aimed to develop microbial production systems of LA and examined LA fermentation conditions. A three-day batch culture produced 200 g/L of LA. A ten-day fed-batch culture with periodic additions of Lac and CaCO₃ accumulated 400 g/L of LA. There was no by-product and no loss of Lac, and the yield of LA-conversion was 100%. The addition of ethanol (75 % (v/v), final concentration) to culture supernatant resulted in recovery of 98 % of LA as precipitate.

収録刊行物

• Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ

  Ｊｏｕｒｎａｌ　ｏｆ　Ａｐｐｌｉｅｄ　Ｇｌｙｃｏｓｃｉｅｎｃｅ 50 (2), 117-120, 2003

  日本応用糖質科学会

キーワード

• lactobionic acid, lactose, Burkholderia cepacia, fermentation, fed-batch culture

詳細情報

• CRID: 1390282681269380352
• NII論文ID: 130002000865
• DOI: 10.5458/jag.50.117
• COI: 1:CAS:528:DC%2BD3sXlsl2qsbs%3D
• ISSN: 18807291
• ISSN: 13447882
• ISSN: http://id.crossref.org/issn/13447882
• Web Site: http://www.jstage.jst.go.jp/article/jag1999/50/2/50_2_117/_pdf
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可