Effects of functional elimination of sarcoplasmic reticulum on the manganese-dependent norepinephrine-induced contractions of the guinea pig vas deferens
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- TSUNOBUCHI-USHIJIMA Hiromi
- Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kanazawa University
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- KATO Hiroshi
- Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kanazawa University
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- UENO Hiroko
- Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kanazawa University
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- GOMI Yasuo
- Department of Pharmacology, Faculty of Pharmaceutical Sciences, Kanazawa University
書誌事項
- タイトル別名
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- Effects of the Functional Elimination of Sarcoplasmic Reticulum on the Manganese-Dependent Norepinephrine-Induced Contractions of the Guinea Pig Vas Deferens.
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We investigated the effects of inhibitors of the sarcoplasmic reticulum (SR) functions on the tonic contractions induced by norepinephrine (NE) in the Ca2+-depleted Mn2+-loaded was deferens of the guinea pig in the absence of both Ca2+ and Mn2+ (Mn2+-dependent NE-contraction). In control preparations without Ca2+ depletion and Mn2+ loading, either cyclopiazonic acid (CPA, 10μM) or ryanodine (RYA, 3μM) inhibited the initial phasic and tonic components but not the large phasic component of NE-induced contraction in normal medium containing 2.2mM Ca2+. In contrast, CPA did not affect the Mn2+-dependent NE-contractions. The inhibitory effect of RYA slowly developed with each repetition of the Mn2+-dependent NE-contraction and the magnitude of the inhibition was slight. A23187 (10μM) inhibited the NE-induced contractions of the control preparations in the same manner as CPA and RYA. Although A23187 did not induce contractions in the Mn2+-loaded preparations, A23187 augmented the Mn2+-dependent NE-contractions. The augmented tonic contractions returned to the resting level by washing NE and A23187. The augmentation remained for 3 successive contractions in the absence of A23187. However, the 2nd application of A23187 did not augment the contraction. These results suggest that neither Mn2+-release from SR nor Mn2+-influx from the extracellular space contributes to the Mn2+-dependent NE-contractions. We concluded that NE induces Mn2+-dependent contractions by increasing Mn2+ sensitivity ofcontractile processes but not by increasing intracellular Mn2+ concentration.
収録刊行物
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- Journal of Smooth Muscle Research
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Journal of Smooth Muscle Research 32 (4), 135-144, 1996
日本平滑筋学会
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詳細情報
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- CRID
- 1390282680032685440
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- NII論文ID
- 130002034945
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- COI
- 1:CAS:528:DyaK28XmsVaksbw%3D
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- ISSN
- 18848796
- 09168737
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- PubMed
- 8910251
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可