<b>Comparative Analysis of MicroRNA-mRNA Expression Profiles of Mesenchymal Stem Cells and Dental Follicle Cells </b>

• Ogura Naomi

  Department of Maxillofacial Surgery Research Institute ofOral Science, Nihon University School ofDentistry at Matsudo, Chiba 271-8587, Japan

• Takahashi Kosuke

  Department of Maxillofacial Surgery

• Iwai Satoshi

  Department of Maxillofacial Surgery

• Fujimoto Yoko

  Department of Maxillofacial Surgery

• Hashimoto Hiroshi

  Department of Maxillofacial Surgery

• Kondoh Toshirou

  Department of Maxillofacial Surgery Research Institute ofOral Science, Nihon University School ofDentistry at Matsudo, Chiba 271-8587, Japan

Bibliographic Information

Other Title

• Comparative Analysis of MicroRNA-mRNA Expression Profiles of Mesenchymal Stem Cells and Dental Follicle Cells

Abstract

Small non-coding microRNAs (miRNAs) regulate cellular and developmental processes through the modulation oftarget gene expression and/or protein synthesis. miRNAs are expressed in a cell-specific manner. Human mesenchymal bone marrow stem cells (hMSC) and human dental follicle cells (hDFC) can undergo multilineage differentiation into various connective tissue cell types, and may therefore be utilized for regeneration in cell therapy. To elucidate the molecular differences that underlie the more primitive characteristics ofhMSC and hDFC, we performed a comprehensive comparative analysis ofthe miRNA and mRNA expression profiles ofhMSC and hDFC using a two-fold change in the normalized intensity of expression as a cut-off to determine differences in expression. In hMSC, 37 were more highly expressed, while 32 miRNAs more highly expressed in hDFC. hMSC expressed high levels ofmiR-196a, -196b, and miR-10a, -10b, which target several homeobox (HOX) genes associated with the patterning ofthe vertebrate axial skeleton. hMSC, but not hDFC, expressed high mRNA levels ofHOX genes. In contrast, miR-199b, which is expressed in tooth germ, as well as the forkhead box O1 (FOXO1) gene, a target ofmiR-1271, were more highly expressed in DFC than in hMSC. The FOXO1 transcription factor plays a role in tooth development and regulates biomineralization. These miRNAs showed specific expression in tissues from which stem cells originate, suggesting that they influence the characteristic mRNA expression of these tissues. These data suggest that analysis of miRNA-mRNA expression profiles is useful for characterization of specific tissue types and cell-specific markers.

Journal

• International Journal of Oral-Medical Sciences

  International Journal of Oral-Medical Sciences 11 (1), 13-21, 2012

  Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo

Keywords

• microRNA, microRNA target gene, mesenchymalstemcells,dentalfollicle cells

Details

• CRID: 1390001205211950336
• NII Article ID: 130002051678
• DOI: 10.5466/ijoms.11.13
• COI: 1:CAS:528:DC%2BC38Xhs1WqtrbO
• ISSN: 21854254
• ISSN: 13479733
• Web Site: https://www.jstage.jst.go.jp/article/ijoms/11/1/11_13/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2013323834
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed