The NP-2 Cell System for Analyses of the Coreceptor Usage of Human and Simian Immunodeficiency Viruses

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Abstract

Human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) are the causative agents of acquired immune deficiency syndrome.Our interests have focused on HIV entry into target cells and HIV celltropism. The cell tropi sm of HIV and the det erm inants of a cell'ssusceptibility to HIV infection have been mainly explained by thecombination of HIV surface proteins, Env, and expression ofCD4 andcoreceptors on the target cells. The coreceptors are molecules belong-ing to the G prot ein-coupled 7-transm embrane receptors, esp eciallychemokine receptors. We propose that many researchers working inthis fi eld have noticed that our sy st em of using a human glioma cellline, NP-2, has be en quite use fu1 for the id entification of HIV corec e-ptors, for the det erm in ation of coreceptor usage in HIV strains and forthe isolation of primary HIV-1 strains, and for the titration of in-fectivity. The properties of the assay systems using NP-2 cells that wehave developed are summarized in this review through an introductionof some of our wotk.

Human immunodeficiency virus type 1 (HIV-1) and type 2 (HIV-2) are the causative agents of acquired immune deficiency syndrome. Our interests have focused on HIV entry into target cells and HIV cell tropism. The cell tropism of HIV and the determinants of a cell's susceptibility to HIV infection have been mainly explained by the combination of HIV surface proteins, Env, and expression of CD4 and coreceptors on the target cells. The coreceptors are molecules belonging to the G protein-coupled 7-transmembrane receptors, especially chemokine receptors. We propose that many researchers working in this field have noticed that our system of using a human glioma cell line, NP-2, has been quite useful for the identification of HIV coreceptors, for the determination of coreceptor usage in HIV strains and for the isolation of primary HIV-1 strains, and for the titration of infectivity. The properties of the assay systems using NP-2 cells that we have developed are summarized in this review through an introduction of some of our work.

Journal

  • The Kitakanto Medical Journal

    The Kitakanto Medical Journal 62(1), 1-14, 2012

    The Kitakanto Medical Society

Codes

  • NII Article ID (NAID)
    130002096501
  • NII NACSIS-CAT ID (NCID)
    AN10585677
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    1343-2826
  • NDL Article ID
    023542440
  • NDL Call No.
    Z19-138
  • Data Source
    NDL  IR  J-STAGE 
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