β-Cryptoxanthin Suppresses the Adipogenesis of 3T3-L1 Cells via RAR Activation
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- SHIRAKURA Yoshiyuki
- Research and Development Center, Unitika Ltd.
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- TAKAYANAGI Katsuhiko
- Research and Development Center, Unitika Ltd.
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- MUKAI Katsuyuki
- Research and Development Center, Unitika Ltd.
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- TANABE Hiroki
- Laboratory of Medicinal Resources, School of Pharmacy, Aichi Gakuin University
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- INOUE Makoto
- Laboratory of Medicinal Resources, School of Pharmacy, Aichi Gakuin University
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Abstract
We recently reported that the oral intake of β-cryptoxanthin exerted anti-obesity effects by lowering visceral fat levels. In the present study, we characterized the molecular mechanisms underlying the lipid-lowering effects of β-cryptoxanthin on 3T3-L1 cells. Consistent with our previous findings, β-cryptoxanthin rapidly reduced the level of intracellular lipids in 3T3-L1 cells as assessed by Oil red O staining. Using an in vitro nuclear receptor binding assay, we demonstrated the ability of β-cryptoxanthin to bind to and activate members of the retinoic acid receptor (RAR) family. Accordingly, treatment of cells with LE540, an RAR antagonist, abolished the β-cryptoxanthin-dependent suppression of 3T3-L1 adipogenesis, suggesting that β-cryptoxanthin mediates its effects on 3T3-L1 cells via RAR activation. In addition, real-time RT-PCR analysis revealed that β-cryptoxanthin down-regulates mRNA expression of PPARγ, a key regulator of adipocyte differentiation, and that this inhibition was blocked by LE540 treatment. Taken together, these data indicate that RAR activation contributes to the molecular mechanism by which β-cryptoxanthin prevents obesity.
Journal
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- Journal of Nutritional Science and Vitaminology
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Journal of Nutritional Science and Vitaminology 57 (6), 426-431, 2011
Center for Academic Publications Japan
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Details 詳細情報について
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- CRID
- 1390001206325715200
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- NII Article ID
- 130002103053
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- NII Book ID
- AA00703822
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- COI
- 1:CAS:528:DC%2BC38Xjt1Kntg%3D%3D
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- ISSN
- 18817742
- 03014800
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- NDL BIB ID
- 023385667
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- PubMed
- 22472285
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
- KAKEN
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- Abstract License Flag
- Disallowed