Preparation of Immunosensors Using a Microfluidic Device with an Interdigitated Array Electrode Modified with Antibodies

• HIRANO Yu

  Research Institute of Genome-based Biofactory, National Institute of Advanced Industrial, Science and Technology (AIST)

• YASUKAWA Tomoyuki

  Graduate School of Material Science, University of Hyogo

• SAWAYASHIKI Yoshihiro

  Graduate School of Environmental Studies, Tohoku University

• SHIKU Hitoshi

  Graduate School of Environmental Studies, Tohoku University

• MIZUTANI Fumio

  Graduate School of Material Science, University of Hyogo

• MATSUE Tomokazu

  Graduate School of Environmental Studies, Tohoku University

Abstract

We prepared a microfluidic device with an interdigitated array electrode modified with antibodies to develop immunosensors. A comb-type array (W1) was used for forming immunocomplexes and another (W2) for detecting mediators generated by the enzyme reaction. We used electropolymerization and avidin-biotin complexes for an addressable immobilization of antibodies on W1. Since the microfluidics significantly accelerated the formation of the immunocomplexes, a period as short as 10 min was sufficient to detect the responses. The current observed at bare array (W2) increased with increasing analyte (mouse IgG) concentration in the range of 1.0–100 ng/mL. Therefore, present procedure is suitable for rapid immunosensing in a simple device.

Journal

• Electrochemistry

  Electrochemistry 78 (2), 175-177, 2010

  The Electrochemical Society of Japan

Keywords

• Microfluidic Device
• Immuno-sensing
• Addressable Immobilization

Details

• CRID: 1390282681472256384
• NII Article ID: 130002150585
• DOI: 10.5796/electrochemistry.78.175
• COI: 1:CAS:528:DC%2BC3cXhvFOiur0%3D
• ISSN: 21862451
• ISSN: 13443542
• Web Site: https://www.jstage.jst.go.jp/article/electrochemistry/78/2/78_2_175/_pdf
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Allowed