LOCAL APPLICATION OF NUCLEUS PULPOSUS INDUCES EXPRESSION OF P2X<sub>3</sub> IN RAT DORSAL ROOT GANGLION CELLS

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Author(s)

    • SATOH KOICHIRO
    • Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine
    • SEKIGUCHI MIHO
    • Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine
    • KIKUCHI SHIN-ICHI
    • Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine
    • KONNO SHIN-ICHI
    • Department of Orthopaedic Surgery, Fukushima Medical University School of Medicine
    • MURAKAWA MASAHIRO
    • Department of Anesthesiology, Fukushima Medical University School of Medicine
    • OLMARKER KJELL
    • Musculoskeletal research, Department of Medical chemistry and Cell biology, University of Gothenburg

Abstract

The P2X<sub>3</sub> receptor is a ligand-gated cation channel that is activated by extra cellular adenosine triphosphate (ATP) found in the dorsal root, trigeminal and nodose ganglia. It is one of the receptors transmitting nociceptive information of injuries and inflammation of the periphery by endogenous ATP released from damaged cells. The present study was performed in order to evaluate if there was an increased expression of P2X<sub>3</sub>-immunoreactivity in dorsal root ganglion (DRG) neurons after experimental disc herniation. There were four groups: exposure of the left L4 dorsal root ganglion and incision of the L4-L5 disc, exposure and slight displacement of the left L4 dorsal ganglion, sham exposure of the L4 dorsal root ganglion, and normal. Seven days after surgery, the DRG's were collected, sectioned and stained immunohistochemically for the P2X<sub>3</sub> receptor. The expression of P2X<sub>3</sub> increased significantly following incision of the L4-5 disc compared to the normal group. Sham surgery induced a minor, although statistically significant increase. Mechanical displacement did not induce any increased expression of the receptors. The study demonstrates that expression of the P2X<sub>3</sub> receptors in the DRG may be induced by local application of nucleus pulposus. This may increase our understanding of the pathophysiologic mechanisms related to disc herniation and sciatica.

Journal

  • FUKUSHIMA JOURNAL OF MEDICAL SCIENCE

    FUKUSHIMA JOURNAL OF MEDICAL SCIENCE 58(1), 17-21, 2012

    The Fukushima Society of Medical Science

Codes

  • NII Article ID (NAID)
    130002153555
  • NII NACSIS-CAT ID (NCID)
    AA0065246X
  • Text Lang
    ENG
  • Article Type
    journal article
  • ISSN
    0016-2590
  • Data Source
    IR  J-STAGE 
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