Effect of Nafamostat on <i>N</i>-Methyl-<small>D</small>-aspartate-Induced Retinal Neuronal and Capillary Degeneration in Rats

• Tsuda Yo

  Department of Molecular Pharmacology, School of Pharmaceutical Sciences, Kitasato University

• Nakahara Tsutomu

  Department of Molecular Pharmacology, School of Pharmaceutical Sciences, Kitasato University

• Ueda Kaori

  Department of Molecular Pharmacology, School of Pharmaceutical Sciences, Kitasato University

• Mori Asami

  Department of Molecular Pharmacology, School of Pharmaceutical Sciences, Kitasato University

• Sakamoto Kenji

  Department of Molecular Pharmacology, School of Pharmaceutical Sciences, Kitasato University

• Ishii Kunio

  Department of Molecular Pharmacology, School of Pharmaceutical Sciences, Kitasato University

Bibliographic Information

Other Title

• Effect of Nafamostat on N-Methyl-D-aspartate-Induced Retinal Neuronal and Capillary Degeneration in Rats

Abstract

We examined the effects of the serine protease inhibitor nafamostat mesilate on neuronal and vascular injury in rat retinas treated with N-methyl-D-aspartate (NMDA). The degree of neuronal degeneration was assessed by measuring the number of cells in the ganglion cell layer and the thickness of the inner plexiform layer. The degree of capillary degeneration was assessed by measuring the number of empty basement membrane sleeves that were left as remnants of the vessels. Significant neuronal and capillary degeneration was observed 7 d after a single intravitreal injection of NMDA into the eye. Both forms of degeneration were significantly prevented by simultaneous injection of nafamostat mesilate with NMDA. These results indicate that nafamostat mesilate affords protection against the neuro/vascular injury seen in NMDA-treated retinas. Nafamostat mesilate may be considered as a candidate for neuro/vascular protective interventions in retinal diseases associated with glutamate-induced excitotoxicity, such as glaucoma and diabetic retinopathy.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 35 (12), 2209-2213, 2012

  The Pharmaceutical Society of Japan

Keywords

• endothelial cell
• excitotoxicity
• retinal blood vessel
• retinal degeneration

Details

• CRID: 1390282679610362240
• NII Article ID: 130002480514
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.b12-00644
• COI: 1:STN:280:DC%2BC3s7oslOksQ%3D%3D
• ISSN: 13475215
• ISSN: 09186158
• NDL BIB ID: 024105746
• PubMed: 23207773
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024105746
• Web Site: https://www.jstage.jst.go.jp/article/bpb/35/12/35_b12-00644/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2013215609
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed