Isolation and Characterization of a Human Intestinal Bacterium <i>Eggerthella</i> sp. CAT-1 Capable of Cleaving the C-Ring of (+)-Catechin and (−)-Epicatechin, Followed by <i>p</i>-Dehydroxylation of the B-Ring
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We isolated a human intestinal bacterium, capable of cleaving the C-ring and dehydroxylating the B-ring of both (+)-catechin (2<i>R</i>,3<i>S</i>) and (−)-epicatechin (2<i>R</i>,3<i>R</i>). Although the strain was classified as <i>Eggerthella</i> (<i>Eg.</i>) <i>lenta</i> [named <i>Eg.</i> sp. CAT-1 (JF798636)] by 16S ribosomal RNA (rRNA) gene similarity, it was quite different in substrate specificity from a previously isolated strain, <i>Eg.</i> sp. SDG-2, which takes part in cleavage of the C-ring and dehydroxylation of the 3,4-dihydroxyphenyl moiety (B-ring) of (3<i>R</i>)-flavan-3-ol derivatives. On the other hand, both <i>Eg.</i> sp. CAT-1 and <i>Eg.</i> sp. SDG-2 showed the same substrate specificity against dehydroxylation of enantiomeric lignans, (+)- and (−)-dihydroxyenterodiol, and (+)- and (−)-dihydroxyenterolactone.
- Biological and Pharmaceutical Bulletin
Biological and Pharmaceutical Bulletin 35(12), 2252-2256, 2012
The Pharmaceutical Society of Japan