Virus-induced silencing of <i>NtmybA1</i> and <i>NtmybA2</i> causes incomplete cytokinesis and reduced shoot elongation in <i>Nicotiana benthamiana</i>

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Author(s)

    • Araki Satoshi
    • Division of Biological Science, Graduate School of Science, Nagoya University
    • Machida Yasunori
    • Division of Biological Science, Graduate School of Science, Nagoya University
    • Ito Masaki
    • Graduate School of Bioagricultural Sciences, Nagoya University

Abstract

In the plant cell cycle, a group of genes with mitotic functions is transcribed specifically during G2 and M phases. Such G2/M-specific genes are regulated by a common mechanism involving a <i>cis</i>-acting element called mitosis-specific activator (MSA) and R1R2R3-class Myb transcription factors that bind to the element. We have previously shown that a group of structurally related R1R2R3-Myb proteins acts as transcriptional activators, which include NtmybA1 and NtmybA2 from tobacco and MYB3R1 and MYB3R4 from <i>Arabidopsis thaliana</i>. Loss-of-function mutations in <i>MYB3R1</i> and <i>MYB3R4</i> showed reduced shoot elongation and significant impairment in cytokinesis during somatic cell division in <i>A. thaliana</i>. However, it remained unclear if this type of Myb transcription factors also have equivalent developmental roles in evolutionarily distant species. Here, we showed virus-induced silencing of both <i>NtmybA1</i> and <i>NtmybA2</i> causes similar defects in cytokinesis and severely dwarfed phenotype. Our results showed that a group of evolutionarily conserved R1R2R3-Myb transcriptional activators has common physiological functions in the promotion of cytokinesis and shoot elongation in different plant species.

Journal

  • Plant Biotechnology

    Plant Biotechnology 29(5), 483-487, 2012

    Japanese Society for Plant Cell and Molecular Biology

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