Plant regeneration from <i>Crotalaria spectabilis</i> hairy roots which showed inhibited growth of root-knot nematodes (<i>Meloidogyne hapla</i> and <i>M. incognita</i>) <i>in vitro</i>

• Ohara Akiko

  Graduate School of Horticulture, Chiba University

• Daimon Hiroyuki

  Graduate School of Life and Environmental Sciences, Osaka Prefecture University

• Momota Yoji

  NARO Institute of Crop Science, National Agriculture and Food Research Organization

• Chin Dong Poh

  Graduate School of Horticulture, Chiba University

• Mii Masahiro

  Graduate School of Horticulture, Chiba University

Bibliographic Information

Other Title

• Plant regeneration from Crotalaria spectabilis hairy roots which showed inhibited growth of root-knot nematodes (Meloidogyne hapla and M. incognita) in vitro

Abstract

Hairy roots were induced from leaf segments of Crotalaria spectabilis, which is used as a green manure crop antagonistic to nematodes, by infection with a mikimopine type wild strain of Agrobacterium rhizogenes A13 (MAFF02-10266). For initial proliferation of hairy roots after induction, addition of gibberellin A₃ to the medium was indispensable. These roots exhibited vigorous growth and abundant lateral branching on half-strength Murashige and Skoog (1/2MS) medium without plant growth regulators. The adventitious shoots were induced from 24% of root segments 2 months after transfer onto B5 medium containing 5 mg l^－1 6-benzyladenine. The axillary buds excised from these shoots produced roots 1 month after transfer onto 0.4% (wv^－1) gellan gum-solidified 1/2MS medium containing 30 mM fructose without plant growth regulators. Regenerated plants were successfully grown under greenhouse conditions. Infection of hairy roots of C. spectabilis with root-knot nematodes Meloidogyne hapla and M. incognita led to the growth inhibition of these nematodes.

Journal

• Plant Biotechnology

  Plant Biotechnology 29 (5), 425-430, 2012

  Japanese Society for Plant Biotechnology

Keywords

• <i>Agrobacterium rhizogenes</i>
• <i>Crotalaria spectabilis</i>
• nematode control
• plant regeneration
• transformation.

Details

• CRID: 1390001204329140224
• NII Article ID: 130002500894
• NII Article ID: 40019545183
• NII Book ID: AA11250821
• DOI: 10.5511/plantbiotechnology.12.0718a
• ISSN: 13476114
• ISSN: 13424580
• NDL BIB ID: 024195405
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024195405
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed