Contraction of Stress Fibers Extracted from Smooth Muscle Cells: Effects of Varying Ionic Strength
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- DEGUCHI Shinji
- Department of Bioengineering and Robotics, Tohoku University
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- MATSUI Tsubasa S.
- Department of Biomolecular Sciences, Tohoku University
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- KOMATSU Daiki
- Department of Bioengineering and Robotics, Tohoku University
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- SATO Masaaki
- Department of Bioengineering and Robotics, Tohoku University Department of Biomedical Engineering, Tohoku University
抄録
Actin stress fibers (SFs) play a key role in regulation of cell adhesion, but the biochemical and biophysical properties intrinsic to SFs remain unclear. Here we extracted SFs from rat embryonic smooth muscle cells by deroofing, and evaluated the effects of varying ionic strength and temperature on their intactness. Wash buffers with ionic strength ranging from 90 to 490 mM were prepared, and the extracted SFs were incubated in a buffer with a particular ionic strength for 10 min or 24 h. Light and electron microscopy revealed that the extracted SFs comprised tightly packed straight bundles at low ionic strengths that became looser and exhibited a ragged pattern at high ionic strengths. The expression of α-actinin associated with the extracted SFs decreased with the increase in ionic strength. Unexpectedly, non-muscle myosin II and smooth muscle myosin in the extracted SFs displayed comparable expression levels over the different ionic strengths. ATP-induced contractility was better preserved at low ionic strengths, including the physiological ionic strength of 170 mM. The rate of ATP-induced enzymatic activity increased with increase in temperature, but the difference was not statistically significant. These results demonstrate that low ionic strength produces extracted SFs that are more intact with regard to structure and function.
収録刊行物
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- Journal of Biomechanical Science and Engineering
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Journal of Biomechanical Science and Engineering 7 (4), 388-398, 2012
一般社団法人 日本機械学会
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詳細情報 詳細情報について
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- CRID
- 1390282680238737920
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- NII論文ID
- 130003322575
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- ISSN
- 18809863
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- Crossref
- CiNii Articles
- KAKEN
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- 抄録ライセンスフラグ
- 使用不可