Good Thermally Conducting Material Supports Follicle Morphologies of Porcine Ovaries Cryopreserved with Ultrarapid Vitrification

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  • HASHIMOTO Shu
    IVF Namba Clinic, Osaka 5500015, Japan School of Biology-Oriented Science and Technology, Kinki University, Wakayama 6496493, Japan St. Marianna University School of Medicine, Kanagawa 2168512, Japan
  • SUZUKI Nao
    St. Marianna University School of Medicine, Kanagawa 2168512, Japan
  • AMO Ami
    IVF Namba Clinic, Osaka 5500015, Japan
  • YAMOCHI Takayuki
    IVF Namba Clinic, Osaka 5500015, Japan
  • HOSOI Yoshihiko
    School of Biology-Oriented Science and Technology, Kinki University, Wakayama 6496493, Japan
  • MORIMOTO Yoshiharu
    IVF Namba Clinic, Osaka 5500015, Japan

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Abstract

Effects of supporting materials during vitrification procedure on the morphologies of preantral follicles of pig ovaries were assessed. Ovarian cortical sections of prepubertal pigs were randomly allocated to 5 groups. The sections were vitrified ultrarapidly with 5 different vitrification devices. The sections were put on 4 fine needles (Cryosupport), on a thin copper plate, or on a carbon graphite sheet or were sandwiched between copper plates or between carbon graphite sheets before cooling. The cooling and warming rates with the graphite sheets were significantly higher than those with the copper plates (P<0.05). A total of 3,064 follicles were analyzed following HE staining after vitrification with 5 different devices. The morphologies follicles vitrified on the Cryosupport or on the graphite sheet were well preserved compared with those vitrified on the copper plate or between copper plates (P<0.01). The morphologies of follicles vitrified between copper plates were mostly damaged (P<0.05). Taken together, good thermally conducting material supports follicle morphologies of ovaries cryopreserved with ultrarapid vitrification.

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