Reassembly of Anterior Pituitary Organization by Hanging Drop Three-Dimensional Cell Culture

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Author(s)

    • Tsukada Takehiro
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Kouki Tom
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Fujiwara Ken
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Ramadhani Dini
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Horiguchi Kotaro
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Kikuchi Motoshi
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine
    • Yashiro Takashi
    • Division of Histology and Cell Biology, Department of Anatomy, Jichi Medical University School of Medicine

Abstract

The anterior pituitary gland comprises 5 types of hormone-producing cells and non-endocrine cells, such as folliculostellate (FS) cells. The cells form a lobular structure surrounded by extracellular matrix (ECM) but are not randomly distributed in each lobule; hormone-producing cells have affinities for specific cell types (topographic affinity), and FS cells form a homotypic meshwork. To determine whether this cell and ECM organization can be reproduced <i>in vitro</i>, we developed a 3-dimensional (3D) model that utilizes hanging drop cell culture. We found that the topographic affinities of hormone-producing cells were indeed maintained (ie, GH to ACTH cells, GH to TSH cells, PRL to LH/FSH cells). Fine structures in hormone-producing cells retained their normal appearance. In addition, FS cells displayed well-developed cytoplasmic protrusions, which interconnected with adjacent FS cells to form a 3D meshwork. In addition, reassembly of gap junctions and pseudofollicles among FS cells was observed in cell aggregates. Major ECM components—collagens and laminin—were deposited and distributed around the cells. In sum, the dissociated anterior pituitary cells largely maintained their <i>in vivo</i> anterior pituitary architectures. This culture system appears to be a powerful experimental tool for detailed analysis of anterior pituitary cell organization.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 46(4), 121-127, 2013

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

Codes

  • NII Article ID (NAID)
    130003361079
  • Text Lang
    ENG
  • ISSN
    0044-5991
  • Data Source
    J-STAGE 
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