<i>In Vitro</i> Evaluation of the Ciliary Beat Frequency of the Rat Nasal Epithelium Using a High-Speed Digital Imaging System

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  • Inoue Daisuke
    Department of Pharmaceutics, School of Pharmacy, Shujitsu University Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Okayama University
  • Furubayashi Tomoyuki
    Department of Pharmaceutics, School of Pharmacy, Shujitsu University
  • Ogawara Ken-ichi
    Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Okayama University
  • Kimura Toshikiro
    Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Okayama University
  • Higaki Kazutaka
    Department of Pharmaceutics, Faculty of Pharmaceutical Sciences, Okayama University
  • Shingaki Tomotaka
    RIKEN Center for Molecular Imaging Science
  • Kimura Shunsuke
    Department of Biopharmaceutics, Kyoto Pharmaceutical University
  • Tanaka Akiko
    Department of Biopharmaceutics, Kyoto Pharmaceutical University
  • Katsumi Hidemasa
    Department of Biopharmaceutics, Kyoto Pharmaceutical University
  • Sakane Toshiyasu
    Department of Biopharmaceutics, Kyoto Pharmaceutical University
  • Yamamoto Akira
    Department of Biopharmaceutics, Kyoto Pharmaceutical University
  • Higashi Yutaka
    Department of Pharmaceutics, School of Pharmacy, Shujitsu University

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  • In Vitro Evaluation of the Ciliary Beat Frequency of the Rat Nasal Epithelium Using a High-Speed Digital Imaging System

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Abstract

Mucociliary clearance (MC) is an important factor in determining nasal drug absorption and the ciliary beat of ciliated epithelial cells of the nasal mucosa is the driving force of MC. However, the relationship between MC and ciliary beat frequency (CBF) is still ambiguous. The purpose of this study was to establish an evaluation method of CBF as an index of mucociliary function and examine the relationship between MC and CBF. A sequence of images of ciliary beating of an excised rat nasal septum was captured using a high-speed digital video camera. CBF (beats per second, Hz) was determined from periodic changes in the contrast value of a specific location in a sequence of images. CBF under control conditions was 8.49±0.38 Hz, which is similar to values reported for cultured human nasal epithelial cells and rat tracheal cells. β-Adrenergic and cholinergic antagonists decreased CBF, while β-adrenergic agonists and acetylcholine increased CBF. These results were similar with those observed for MC in our previous study. It was found that CBFs were significantly and linearly correlated with MC, indicating that MC is directly regulated by CBF and that this evaluation system allows the quantitative determination of nasal mucociliary function.

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