Neuroprotective Effects of Curcumin and Highly Bioavailable Curcumin on Oxidative Stress Induced by Sodium Nitroprusside in Rat Striatal Cell Culture

• Nazari Qand Agha

  Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University

• Kume Toshiaki

  Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University

• Izuo Naotaka

  Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University

• Takada-Takatori Yuki

  Department of Pharmacology, Faculty of Pharmaceutical Sciences, Doshisha Women’s College

• Imaizumi Atsushi

  Theravalues Corporation

• Hashimoto Tadashi

  Theravalues Corporation

• Izumi Yasuhiko

  Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University

• Akaike Akinori

  Department of Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University Graduate School of Pharmaceutical Sciences, Nagoya University

Abstract

Curcumin, a polyphenolic compound extracted from Curcuma longa, has several pharmacological activities such as anticancer, anti-inflammatory, and antioxidant effects. The purpose of this study was to investigate the protective effects of curcumin and THERACURMIN, a highly bioavailable curcumin, against sodium nitroprusside (SNP)-induced oxidative damage in primary striatal cell culture. THERACURMIN as well as curcumin significantly prevented SNP-induced cytotoxicity. To elucidate the cytoprotective effects of curcumin and THERACURMIN, we measured the intracellular glutathione level in striatal cells. Curcumin and THERACURMIN significantly elevated the glutathione level, which was decreased by treatment with SNP. Moreover, curcumin showed potent 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging ability. Finally, a ferrozine assay showed that curcumin (10–100 µg/mL) has potent Fe²⁺-chelating ability. These results suggest that curcumin and THERACURMIN exert potent protective effects against SNP-induced cytotoxicity by free radical-scavenging and iron-chelating activities.

Journal

• Biological and Pharmaceutical Bulletin

  Biological and Pharmaceutical Bulletin 36 (8), 1356-1362, 2013

  The Pharmaceutical Society of Japan

Keywords

• curcumin
• THERACURMIN
• sodium nitroprusside
• oxidative stress

Details

• CRID: 1390282679610400000
• NII Article ID: 130003361498
• NII Book ID: AA10885497
• DOI: 10.1248/bpb.b13-00300
• COI: 1:STN:280:DC%2BC3sflslWlug%3D%3D
• ISSN: 13475215
• ISSN: 09186158
• NDL BIB ID: 024716079
• PubMed: 23902979
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024716079
• Web Site: https://www.jstage.jst.go.jp/article/bpb/36/8/36_b13-00300/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2014095524
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed