LSI/FABを用いる多官能性ヌクレオシドホスホロアミダイトのマススペクトル測定

• 藤嶽 美穂代

  大阪薬科大学MS室

• 春沢 信哉

  大阪薬科大学有機薬化学研究室

書誌事項

タイトル別名

• Mass Spectrometric Determination of Polyfunctionalized Nucleoside Phosphoramidites Using LSI/FAB
• LSI/FAB オ モチイル タカンノウセイ ヌクレオシドホスホロアミダイト ノ マススペクトル ソクテイ

抄録

  Nucleoside phosphoramidites (PAs) are the most widely used building blocks in the contemporary solid-phase synthesis of oligonucleotides. Accurate molecular weight measurements of such acid-labile compounds may be easily determined in a matrix system comprising triethanolamine (TEOA)-NaCl by liquid secondary ionization mass spectrometry (LSIMS) or fast atom bombardment mass spectrometry (FABMS), using a double-focusing mass spectrometer. The present method rapidly and easily measures the accurate MWs of various PAs as adduct ions [M+Na]⁺, affording molecular formulas in place of elemental analysis. Further, the intensities by LSIMS of [M+Na]⁺ were found to be enhanced to the highest degree by adjustment of the mole ratio of PA and NaCl, fixing the amount of TEOA. In addition, the effects of the metal ion in the matrix were investigated. The present method is a powerful tool for the mass spectral identification of polyfunctionalized nucleoside PAs.<br>

収録刊行物

• 薬学雑誌

  薬学雑誌 133 (7), 823-841, 2013-07-01

  公益社団法人 日本薬学会

キーワード

• molecular weight
• nucleoside phosphoramidite
• matrix
• mass spectrometry (MS)

詳細情報

• CRID: 1390001206127593344
• NII論文ID: 130003361966
• NII書誌ID: AN00284903
• DOI: 10.1248/yakushi.13-00097
• COI: 1:STN:280:DC%2BC3sjmsVOhuw%3D%3D
• ISSN: 13475231
• ISSN: 00316903
• NDL書誌ID: 024785618
• PubMed: 23811770
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I024785618
• Web Site: https://www.jstage.jst.go.jp/article/yakushi/133/7/133_13-00097/_pdf
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
  • KAKEN
• 抄録ライセンスフラグ: 使用不可