Cross-Reactivity of Secondary Antibodies against African Rodents and Application for Sero-Surveillance

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Author(s)

    • NAKAMURA Ichiro NAKAMURA Ichiro
    • Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan|School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia
    • SAWA Hirofumi [他] TAKADA Ayato
    • Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan|School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia
    • SUGIMOTO Chihiro
    • Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan|School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia
    • ARIKAWA Jiro
    • Graduate School of Medicine, Hokkaido University, N15W7, Kita-ku, Sapporo, Hokkaido 060–8638, Japan
    • SAWA Hirofumi
    • Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan|School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia
    • KOBAYASHI Shintaro
    • Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan
    • ORBA Yasuko
    • Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan
    • ISHII Akihiro
    • Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan|School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia
    • THOMAS Yuka
    • Hokkaido University Research Center for Zoonosis Control, N20W10, Kita-ku, Sapporo, Hokkaido 001–0020, Japan|School of Veterinary Medicine, University of Zambia, P. O. Box 32379, Lusaka, Zambia
    • ISOZUMI Rie
    • Graduate School of Medicine, Hokkaido University, N15W7, Kita-ku, Sapporo, Hokkaido 060–8638, Japan
    • YOSHIMATSU Kumiko
    • Graduate School of Medicine, Hokkaido University, N15W7, Kita-ku, Sapporo, Hokkaido 060–8638, Japan

Abstract

A total of 466 rodents were captured in the Republic of Zambia from 2006 to 2010. Based on morphological observations and phylogenetic analyses of mitochondrial gene sequences, rodents were divided into 10 groups consisting of 39 <i>Rattus</i> rodents, 263 multimammate rats, 18 other Murinae rodents, 95 gerbils, 11 pouched mice, 1 giant-pouched rat, 38 fat mice and 1 dormouse. Rodent antibodies except that from <i>Rattus</i> were examined for their cross-reactivity to commercially available antibody detection reagents. Anti-mouse immunoglobulin G (IgG) was most cross-reactive to heterologous antibodies including multimammate rat, gerbil, pouched mouse and fat mouse. Thus, anti-mouse IgG would be a useful detection tool in serological examination of the Zambian rodent population. Preliminary sero-surveillance for plague, leptospirosis and hantavirus infection was performed by ELISA.

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 75(6), 819-825, 2013

    JAPANESE SOCIETY OF VETERINARY SCIENCE

Codes

  • NII Article ID (NAID)
    130003362128
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • ISSN
    0916-7250
  • NDL Article ID
    024734604
  • NDL Call No.
    Z18-350
  • Data Source
    NDL  J-STAGE 
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