Cross-Reactivity of the Anti-Human D-dimer Monoclonal Antibody 1C9-6F10 to Canine Fibrin Degradation Products

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Author(s)

    • MIURA Naoki MIURA Naoki
    • Laboratory of Diagnostic Imaging, Department of Veterinary Science, Faculty of Agriculture, Kagoshima University, 1–21–24 Korimoto, Kagoshima 890–0065, Japan
    • FURUKAWA Makoto FURUKAWA Makoto
    • Laboratory of Diagnostic Imaging, Department of Veterinary Science, Faculty of Agriculture, Kagoshima University, 1–21–24 Korimoto, Kagoshima 890–0065, Japan
    • MOMOI Yasuyuki
    • Laboratory of Diagnostic Imaging, Department of Veterinary Science, Faculty of Agriculture, Kagoshima University, 1–21–24 Korimoto, Kagoshima 890–0065, Japan

Abstract

The cross-reactivity of the anti-human D-dimer antibody 1C9-6F10 to the canine D-dimer was evaluated by western blotting using purified canine fibrinogen, fibrin degradation products (XDPs) and plasma from a dog with suspected disseminated intravascular coagulation (DIC). The antibody cross-reacted with canine XDPs and plasma from the dog with suspected DIC. A latex agglutination assay using 1C9-6F10 measured the canine XDPs in dogs. In conclusion, the antibody 1C9-6F10 cross-reacted to the canine D-fragment with high specificity, but lower affinity compared with the human D-dimer. The latex agglutination assay using the 1C9-6F10 antibody was used to measure canine plasma D-dimer concentrations.

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 75(7), 963-966, 2013

    JAPANESE SOCIETY OF VETERINARY SCIENCE

Codes

  • NII Article ID (NAID)
    130003362135
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • ISSN
    0916-7250
  • NDL Article ID
    024819763
  • NDL Call No.
    Z18-350
  • Data Source
    NDL  J-STAGE 
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