Accumulation of Xenotransplanted Canine Bone Marrow Cells in NOD/SCID/γc[null] Mice with Acute Hepatitis Induced by CCl₄
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- KATO Takashi
- Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
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- HISASUE Masaharu
- Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
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- SEGAWA Kazuhito
- Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
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- FUJIMOTO Ayumi
- Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
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- MAKIISHI Eri
- Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
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- NEO Sakurako
- Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
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- YASUNO Kyohei
- Research Institute of Biosciences, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
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- KOBAYASHI Ryosuke
- Research Institute of Biosciences, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
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- TSUCHIYA Ryo
- Laboratory of Internal Medicine II, School of Veterinary Medicine, Azabu University, 1–17–71 Fuchinobe, Chuoku, Sagamihara, Kanagawa 252–5201, Japan
書誌事項
- タイトル別名
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- Accumulation of Xenotransplanted Canine Bone Marrow Cells in NOD/SCID/γ<sub>c</sub><sup>null</sup> Mice with Acute Hepatitis Induced by CCl<sub>4</sub>
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抄録
Bone marrow cell infusion (BMI) has recently been suggested as an effective therapy for refractory liver disease; however, the efficiency of BMI using canine bone marrow cells (cBMCs) has not been reported. We evaluated the accumulation potential of cBMCs in a mouse model of acute liver failure. Acute hepatitis was induced by carbon tetrachloride (CCl4) treatment in NOD/SCID/γcnull(NOG) mice and wild-type (WT) C57BL mice, and the characteristics of liver dysfunction and the degree of hepatic injury and regeneration were compared between the two mouse models. Next, female CCl4-treated NOG mice were xenotransplanted with male PKH26-labeled cBMCs, and the potential of cBMCs to accumulate in injured liver tissue compartments was examined. Fluorescence microscopy was performed to histologically detect the infused cBMCs, and DNA polymerase chain reaction was performed for detection of the male Y chromosome (SRY gene) in the recipient female NOG mice. The number of PKH26-positive cBMCs transplanted in the liver tissue gradually increased in the NOG mice. The infused cBMCs were located in the necrotic area of the liver at an early stage after transplantation, and most had accumulated a week after transplantation. However, the therapeutic efficacy of the xenotransplantation remained unclear, because no significant differences were observed concerning the extent liver injury and regeneration between the cBMC-transplanted and saline control mice. These results suggest that cBMCs will specifically accumulate in injured liver tissue and that BMC transplantation may have the potential to repair liver deficiency.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 75 (7), 847-855, 2013
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681406803968
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- NII論文ID
- 130003362149
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- NII書誌ID
- AA10796138
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- COI
- 1:STN:280:DC%2BC3szosFCisg%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 024819528
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- PubMed
- 23411484
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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