Multilocus Sequence Typing and DNA Similarity Analysis Implicates that a <i>Borrelia valaisiana</i>–related sp. Isolated in Japan is Distinguishable from European <i>B. valaisiana</i>

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Author(s)

    • TAKADA Nobuhiro
    • Department of Pathological Sciences, Faculty of Medical Sciences, University of Fukui, Fukui 910–1193, Japan
    • YANO Yasuhiro
    • Department of Pathological Sciences, Faculty of Medical Sciences, University of Fukui, Fukui 910–1193, Japan
    • ANDOH Masako
    • Department of Virology I, National Institute of Infectious Diseases, Tokyo 162–8640, Japan
    • ANDO Shuji
    • Department of Virology I, National Institute of Infectious Diseases, Tokyo 162–8640, Japan
    • SATO Kozue
    • Department of Bacteriology-I, National Institute of Infectious Diseases, Tokyo 162–8640, Japan
    • TAKAHASHI Hideyuki
    • Department of Bacteriology-I, National Institute of Infectious Diseases, Tokyo 162–8640, Japan
    • OHNISHI Makoto
    • Department of Bacteriology-I, National Institute of Infectious Diseases, Tokyo 162–8640, Japan
    • TAKANO Ai
    • Department of Bacteriology-I, National Institute of Infectious Diseases, Tokyo 162–8640, Japan|Joint Faculty of Veterinary Medicine, Yamaguchi University, 1677–1 Yoshida, Yamaguchi 753–8515, Japan
    • KADOSAKA Teruki
    • Department of Infection and Immunity, Aichi Medical University, Aichi 480–1195, Japan
    • FUJITA Hiromi
    • Mahara Institute of Medical Acarology, Tokushima 779–1510, Japan
    • NITTA Yoshiki
    • Okinawa Prefectural Institute of Animal Health, Okinawa 900–0024, Japan
    • GOKUDEN Mutsuyo
    • Kagoshima Prefectural Institute of Environmental Research and Public Health, Kagoshima 892–0853, Japan
    • HONDA Toshiro
    • Kagoshima Prefectural Institute of Environmental Research and Public Health, Kagoshima 892–0853, Japan|Kaseda Public Health Center, Kagoshima 897–0001, Japan
    • TOMIDA Junko
    • School of Pharmacy, Aichi Gakuen University, Aichi 464–8650, Japan

Abstract

Lyme disease <i>Borrelia</i> spp. are transmitted by <i>Ixodes</i> ticks, and more than 10 species of borreliae have been identified around the world. Recently, another <i>Borrelia</i> sp. has been reported in Asia (Japan, Korea, China, Taiwan and Thailand) as <i>Borrelia valaisiana</i>-related sp. In the present study, we obtained and genetically characterized 19 <i>B. valaisiana</i>-related sp. strains from mammals and ticks. Genetic analyses showed that the <i>Borrelia</i> strains were distinct from <i>B. valaisiana</i> found in Europe. Multilocus sequence typing revealed that these <i>Borrelia</i> isolates formed a monophyletic group with <i>B. yangtze</i> strains in China. Some of the strains were isolated from the bladders of small mammals, and also two strains were experimentally confirmed to be infectious in C3H/HeN mice. We observed that the <i>Borrelia</i> sp. was maintained in the <i>Ixodes granulatus</i> tick after molting. These results suggested that small mammals and <i>I. granulatus</i> were possible reservoir hosts and the vector tick for the <i>Borrelia</i> sp., respectively. <i>B. valaisiana</i>, originally found in Europe, was transmitted mainly by <i>I. ricinus</i>, and birds were mainly thought to be reservoir hosts. Our results suggested that Japanese isolates of <i>B. yangtze </i>(formerly <i>B. valaisiana</i>-related sp.) were distinguishable from <i>B. valaisiana</i> according to the reservoir host and its vector tick. In this study, we also deposited borrelia strain Okinawa-CW62 into bioresource centers as a reference strain of <i>B. yangtze</i>(=DSM 24625, JCM 17189).

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 75(9), 1201-1207, 2013

    JAPANESE SOCIETY OF VETERINARY SCIENCE

Codes

  • NII Article ID (NAID)
    130003362209
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • ISSN
    0916-7250
  • NDL Article ID
    024906864
  • NDL Call No.
    Z18-350
  • Data Source
    NDL  J-STAGE 
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