Multilocus Sequence Typing and DNA Similarity Analysis Implicates that a <i>Borrelia valaisiana</i>–related sp. Isolated in Japan is Distinguishable from European <i>B. valaisiana</i>
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Lyme disease <i>Borrelia</i> spp. are transmitted by <i>Ixodes</i> ticks, and more than 10 species of borreliae have been identified around the world. Recently, another <i>Borrelia</i> sp. has been reported in Asia (Japan, Korea, China, Taiwan and Thailand) as <i>Borrelia valaisiana</i>-related sp. In the present study, we obtained and genetically characterized 19 <i>B. valaisiana</i>-related sp. strains from mammals and ticks. Genetic analyses showed that the <i>Borrelia</i> strains were distinct from <i>B. valaisiana</i> found in Europe. Multilocus sequence typing revealed that these <i>Borrelia</i> isolates formed a monophyletic group with <i>B. yangtze</i> strains in China. Some of the strains were isolated from the bladders of small mammals, and also two strains were experimentally confirmed to be infectious in C3H/HeN mice. We observed that the <i>Borrelia</i> sp. was maintained in the <i>Ixodes granulatus</i> tick after molting. These results suggested that small mammals and <i>I. granulatus</i> were possible reservoir hosts and the vector tick for the <i>Borrelia</i> sp., respectively. <i>B. valaisiana</i>, originally found in Europe, was transmitted mainly by <i>I. ricinus</i>, and birds were mainly thought to be reservoir hosts. Our results suggested that Japanese isolates of <i>B. yangtze </i>(formerly <i>B. valaisiana</i>-related sp.) were distinguishable from <i>B. valaisiana</i> according to the reservoir host and its vector tick. In this study, we also deposited borrelia strain Okinawa-CW62 into bioresource centers as a reference strain of <i>B. yangtze</i>(=DSM 24625, JCM 17189).
- Journal of Veterinary Medical Science
Journal of Veterinary Medical Science 75(9), 1201-1207, 2013
JAPANESE SOCIETY OF VETERINARY SCIENCE