LC-MS/MS Analysis of Canine Lipoproteins Fractionated Using the Ultracentrifugation-Precipitation Method
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- SUTO Asuka
- Department of Dermatology, Hokkaido University Graduate School of Medicine, Kita15 Nishi7, Kita-ku, Sapporo, Hokkaido 060–8638, Japan Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita18 Nishi9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
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- YAMASAKI Masahiro
- Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita18 Nishi9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
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- TAKASAKI Yukari
- Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita18 Nishi9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
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- FUJITA Yasuyuki
- Department of Dermatology, Hokkaido University Graduate School of Medicine, Kita15 Nishi7, Kita-ku, Sapporo, Hokkaido 060–8638, Japan
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- ABE Riichiro
- Department of Dermatology, Hokkaido University Graduate School of Medicine, Kita15 Nishi7, Kita-ku, Sapporo, Hokkaido 060–8638, Japan
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- SHIMIZU Hiroshi
- Department of Dermatology, Hokkaido University Graduate School of Medicine, Kita15 Nishi7, Kita-ku, Sapporo, Hokkaido 060–8638, Japan
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- OHTA Hiroshi
- Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita18 Nishi9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
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- TAKIGUCHI Mitsuyoshi
- Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita18 Nishi9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
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抄録
Due to the lack of a gold standard method in canine lipoprotein analysis, it is unclear whether canine high-density lipoprotein (HDL) and low-density lipoprotein (LDL) can be accurately evaluated by the lipoprotein analysis methods used for dogs. This study investigated whether the ultracentrifugation-precipitation (U-P) method was suitable as a gold standard method for analyzing canine lipoprotein. First, the U-P method was compared with a gel permeation high-performance liquid chromatography system (GP-HPLC). The concentrations of canine HDL cholesterol (HDL-C) and LDL cholesterol (LDL-C) determined by the U-P method correlated closely with those determined by GP-HPLC. However, the canine HDL-C concentration determined by the U-P method was lower than that determined by GP-HPLC, and the canine LDL-C concentration determined by the U-P method was higher than that determined by GP-HPLC. This study showed that some canine HDL could be precipitated with heparin manganese chloride solution. Second, the HDL and LDL fractions separated by the U-P method were analyzed by LC-MS/MS. The HDL fraction was found to contain only apolipoprotein A-I, which is an apolipoprotein of HDL, whereas the LDL fraction contained both apolipoprotein A-I and apolipoprotein B-100, which is an apolipoprotein of LDL. This data showed that a certain lipoprotein that includes apolipoprotein A-I might precipitate with canine LDL when using heparin manganese chloride solution. These results indicated that the U-P method is not currently a gold standard method for analyzing canine lipoproteins.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 75 (11), 1471-1477, 2013
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681404640768
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- NII論文ID
- 130003362254
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- NII書誌ID
- AA10796138
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- COI
- 1:STN:280:DC%2BC3sfgvFGhtA%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 025039770
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- PubMed
- 23856814
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可