LC-MS/MS Analysis of Canine Lipoproteins Fractionated Using the Ultracentrifugation-Precipitation Method

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Author(s)

    • SUTO Asuka SUTO Asuka
    • Department of Dermatology, Hokkaido University Graduate School of Medicine, Kita15 Nishi7, Kita-ku, Sapporo, Hokkaido 060–8638, Japan|Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita18 Nishi9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
    • YAMASAKI Masahiro YAMASAKI Masahiro
    • Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita18 Nishi9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
    • TAKASAKI Yukari [他] TAKASAKI Yukari
    • Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita18 Nishi9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
    • FUJITA Yasuyuki
    • Department of Dermatology, Hokkaido University Graduate School of Medicine, Kita15 Nishi7, Kita-ku, Sapporo, Hokkaido 060–8638, Japan
    • ABE Riichiro
    • Department of Dermatology, Hokkaido University Graduate School of Medicine, Kita15 Nishi7, Kita-ku, Sapporo, Hokkaido 060–8638, Japan
    • SHIMIZU Hiroshi
    • Department of Dermatology, Hokkaido University Graduate School of Medicine, Kita15 Nishi7, Kita-ku, Sapporo, Hokkaido 060–8638, Japan
    • OHTA Hiroshi
    • Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita18 Nishi9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan
    • TAKIGUCHI Mitsuyoshi
    • Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Kita18 Nishi9, Kita-ku, Sapporo, Hokkaido 060–0818, Japan

Abstract

Due to the lack of a gold standard method in canine lipoprotein analysis, it is unclear whether canine high-density lipoprotein (HDL) and low-density lipoprotein (LDL) can be accurately evaluated by the lipoprotein analysis methods used for dogs. This study investigated whether the ultracentrifugation-precipitation (U-P) method was suitable as a gold standard method for analyzing canine lipoprotein. First, the U-P method was compared with a gel permeation high-performance liquid chromatography system (GP-HPLC). The concentrations of canine HDL cholesterol (HDL-C) and LDL cholesterol (LDL-C) determined by the U-P method correlated closely with those determined by GP-HPLC. However, the canine HDL-C concentration determined by the U-P method was lower than that determined by GP-HPLC, and the canine LDL-C concentration determined by the U-P method was higher than that determined by GP-HPLC. This study showed that some canine HDL could be precipitated with heparin manganese chloride solution. Second, the HDL and LDL fractions separated by the U-P method were analyzed by LC-MS/MS. The HDL fraction was found to contain only apolipoprotein A-I, which is an apolipoprotein of HDL, whereas the LDL fraction contained both apolipoprotein A-I and apolipoprotein B-100, which is an apolipoprotein of LDL. This data showed that a certain lipoprotein that includes apolipoprotein A-I might precipitate with canine LDL when using heparin manganese chloride solution. These results indicated that the U-P method is not currently a gold standard method for analyzing canine lipoproteins.

Journal

  • Journal of Veterinary Medical Science

    Journal of Veterinary Medical Science 75(11), 1471-1477, 2013

    JAPANESE SOCIETY OF VETERINARY SCIENCE

Codes

  • NII Article ID (NAID)
    130003362254
  • NII NACSIS-CAT ID (NCID)
    AA10796138
  • Text Lang
    ENG
  • ISSN
    0916-7250
  • NDL Article ID
    025039770
  • NDL Call No.
    Z18-350
  • Data Source
    NDL  J-STAGE 
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