Establishment and Application of a Loop-Mediated Isothermal Amplification Method for Simple, Specific, Sensitive and Rapid Detection of Toxoplasma gondii
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- CAO Lili
- Jilin Academy of Animal Husbandry and Veterinary Medicine, Changchun, Jilin 130062, China
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- CHENG Ronghua
- Jilin Academy of Animal Husbandry and Veterinary Medicine, Changchun, Jilin 130062, China
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- YAO Lin
- College of Agriculture, Yanbian University, Yanji, Jilin 133002, China
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- YUAN Shuxian
- Jilin Academy of Animal Husbandry and Veterinary Medicine, Changchun, Jilin 130062, China
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- YAO Xinhua
- Jilin Academy of Animal Husbandry and Veterinary Medicine, Changchun, Jilin 130062, China
書誌事項
- タイトル別名
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- Establishment and Application of a Loop-Mediated Isothermal Amplification Method for Simple, Specific, Sensitive and Rapid Detection of <i>Toxoplasma gondii</i>
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抄録
The Loop-mediated isothermal amplification (LAMP) method amplifies DNA with high simply, specificity, sensitivity and rapidity. In this study, A LAMP assay with 6 primers targeting a highly conserved region of the GRA1 gene was developed to diagnose Toxoplasma gondii. The reaction time of the LAMP assay was shortened to 30 min after optimizing the reaction system. The LAMP assay was found to be highly specific and stable. The detection limit of the LAMP assay was 10 copies, the same as that of the conventional PCR. We used the LAMP assay to develop a real-time fluorogenic protocol to quantitate T. gondii DNA and generated a log-linear regression plot by plotting the time-to-threshold values against genomic equivalent copies. Furthermore, the LAMP assay was applied to detect T. gondii DNA in 423 blood samples and 380 lymph node samples from 10 pig farms, and positive results were obtained for 7.8% and 8.2% of samples, respectively. The results showed that the LAMP method is slightly more sensitive than conventional PCR (6.1% and 7.6%). Positive samples obtained from 6 pig farms. The LAMP assay established in this study resulted in simple, specific, sensitive and rapid detection of T. gondii DNA and is expected to play an important role in clinical detection of T. gondii.
収録刊行物
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- The Journal of Veterinary Medical Science
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The Journal of Veterinary Medical Science 76 (1), 9-14, 2014
公益社団法人 日本獣医学会
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詳細情報 詳細情報について
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- CRID
- 1390282681405788544
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- NII論文ID
- 130003362283
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- NII書誌ID
- AA10796138
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- COI
- 1:STN:280:DC%2BC3sbhvVGhsw%3D%3D
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- ISSN
- 13477439
- 09167250
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- NDL書誌ID
- 025172385
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- PubMed
- 23965849
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可