Whipple Disease Diagnosed with PCR Using Formalin-fixed Paraffin-embedded Specimens of the Intestinal Mucosa
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- Yajima Nobuhisa
- Department of Pathology and Molecular Medicine, Hirosaki University Graduate School of Medicine, Japan
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- Wada Ryuichi
- Department of Pathology and Molecular Medicine, Hirosaki University Graduate School of Medicine, Japan
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- Kimura Sotai
- Department of Pathology and Molecular Medicine, Hirosaki University Graduate School of Medicine, Japan
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- Matsuzaki Yutaka
- Department of Pathology and Molecular Medicine, Hirosaki University Graduate School of Medicine, Japan
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- Chiba Daisuke
- Department of Internal Medicine, Kensei Hospital, Japan
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- Ebina Yoshihito
- Department of Pathology, Kensei Hospital, Japan
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- Ohkusu Kiyofumi
- Department of Microbiology, Regeneration and Advanced Medical Science, Gifu University Graduate School of Medicine, Japan
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- Yagihashi Soroku
- Department of Pathology and Molecular Medicine, Hirosaki University Graduate School of Medicine, Japan
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抄録
We herein present the case of a 54-year-old Japanese woman with Whipple disease diagnosed with polymerase chain reaction (PCR) using formalin-fixed paraffin-embedded (FFPE) specimens. The patient complained of weight loss, diarrhea and arthralgia. An endoscopic examination revealed swollen villi in the duodenum and ileum. Pathology demonstrated the presence of numerous macrophages filled with diastase-resistant PAS-positive particles. PCR using FFPE specimens amplified a fragment of 16S rDNA from Tropheryma whipplei. After the administration of ceftriaxone followed by trimethoprim/sulfamethoxazole, no signs of recurrence were observed for two years. The use of FFPE specimens for PCR should be considered for the prompt diagnosis and prevention of disease progression.<br>
収録刊行物
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- Internal Medicine
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Internal Medicine 52 (2), 219-222, 2013
一般社団法人 日本内科学会
