有茎分節腸管内肝組織片充填式補助肝臓の開発―第1報  [in Japanese] Development of an Auxiliary Liver Using a Small Intestinal Segment Packed with Liver Microfragments  [in Japanese]

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Abstract

<B>【背景・目的】</B> 肝不全の治療には肝移植が効果的な方法であるがドナー不足の現状がある. 本研究はドナー不足を補えるような効果的な補助肝臓の開発を目指している. <B>【対象と方法】</B> 雄性ウイスター系ラット300-400gを用いた. 2-3 cmの分節空腸をその栄養動静脈を温存して単離する. その粘膜を削除した中に同じラットから摘出した左葉をミンチし, これを充填して補助肝臓を作成した. 決められた期間に犠牲死させ腸管グラフト内の肝組織片の状態を組織学的に検討した. <B>【結 果】</B> 移植後10日までは充填した肝組織片はお互いに融合し肝の小葉構造を形成して増殖を続けたがそれ以降になると次第に壊死融解する部分が大きくなった. <B>【結 語】</B> 腸管グラフト内への肝組織片移植法は補助肝臓として有望であると考えられたが肝組織片の増殖, 増大に伴う血流の維持をどのように計るのかが今後の課題である.

<B>Objective : </B>Liver transplantation is an effective method for the treatment of hepatic failure, but donor shortage remains problematic. This study was conducted to develop an effective auxiliary liver capable of compensating for the donor shortage. <B>Design : </B>Male Wistar rats (300-400g) were used. A jejunal segment (2-3 cm) was isolated together with a feeding pedicle. After the removal of the mucosa, the intestinal segment was filled with liver microfragments obtained from the left liver of the same individual rat. Groups of 3-5 rats were killed at various intervals and the viability of the liver graft was analyzed histologically. <B>Results : </B>Within 10 days after transplantation, the confluence of the transplanted liver tissue was restored and the tissue exhibited a normal hepatic architecture. However, the confluent and enlarged liver tissue gradually became necrotic, and nearly 70% of the liver tissue had been lost at 40 days after transplantation. After 10 months, almost all the liver tissue had become necrotic, although the intestinal segment remained viable. <B>Conclusions : </B>This method has the potential to construct an effective auxiliary liver ; however, further improvement to obtain sufficient blood flow will be required to maintain the transplanted liver tissue.

Journal

  • The Kitakanto Medical Journal

    The Kitakanto Medical Journal 63(2), 133-140, 2013

    The Kitakanto Medical Society

Codes

  • NII Article ID (NAID)
    130003368238
  • NII NACSIS-CAT ID (NCID)
    AN10585677
  • Text Lang
    JPN
  • Article Type
    Article
  • ISSN
    1343-2826
  • NDL Article ID
    024745880
  • NDL Call No.
    Z19-138
  • Data Source
    NDL  IR  J-STAGE 
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