Gene Expression Profiling during Growth in vitro Using a Custom-made Porphyromonas gingivalis Gene Array

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  • Hiratsuka Koichi
    Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Matsudo, Chiba 271-8587, Japan
  • Abiko Yoshimitsu
    Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo Research Institute of Oral Science, Matsudo, Chiba 271-8587, Japan

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  • <b>Gene Expression Profiling during Growth </b><b><i>in vitro </i></b><b>Using a Custom-made </b><b><i>Porphyromonas gingivalis </i></b><b>Gene Array </b>

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A custom-made microarray for Porphyromonas gingivalis was constructed to investigate the gene expression profiling during its in vitro growth. There was a strong positive correlation between the results of both microarray and quantitative real-timePCR analyses (r=0.910, p<0.01). Many transcripts changed dramatically during the late-log to theearly stationary phase. In a stationary phase, most of the transcripts decreased. However, transcripts related to the transposon function, oxidative stress, and energy metabolism were increased or sustained at high levels. More notably, the transcripts related to protein folding and protein stabilization, such as dnak, groEL, groES, and htpG, increased significantly after 10h in the stationary phase, suggesting that these chaperone proteins may play an important role in bacterial survival. All together, our custom array is useful for transcriptional research for P. gingivalis. It provides that the gene expressions are growth phase-specific and are changed dramatically in response to environmental change during bacterial culture.

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  • ＩＪＯＭＳ

    ＩＪＯＭＳ 11 (3), 141-150, 2013

    日本大学松戸歯学部 口腔科学研究所

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