<b>Microarray Analysis Detection of Signaling Pathway Responsive to IL-1β in Synovial Fibroblasts from TMJ </b>

• Kishida Tsuyoshi

  Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan

• Ogura Naomi

  Department of Maxillofacial Surgery, Nihon University School of Dentistry at Matsudo, Matsudo, Chiba 271-8587, Japan

Bibliographic Information

Other Title

• Microarray Analysis Detection of Signaling Pathway Responsive to IL-1β in Synovial Fibroblasts from TMJ

Abstract

Interleukin-1β (IL-1β) has important roles in the inflammation and connective tissue destruction observed in joint diseases such as rheumatoid arthritis. IL-1β is also a key mediator of intracapsular pathologic conditions of the temporomandibular joint (TMJ),including disk displacement/internal derangement and osteoarthritis. To identify putative IL-1β-responsive genes from arthritic disease tissues, gene expression of IL-1β treated and untreated synovial fibroblasts was measured using DNA microarray, and IL-1β-responsive genes were analyzed with GeneSpring. To measure the expression of 8,793 genes in synovial fibroblasts from five TMJ patients, significant changes between controls and IL-1β-treated cells (p<0.05) were detected in 170 genes; 139 up-regulated genes, and 31 down-regulated genes. In addition, the biological interactions of IL-1β-regulated genes were investigated using Ingenuity Pathway Analysis, and it was found that IL-1β affects the expression of several genes in the NFκB signaling pathway. NFKB1 gene expression increased in synovial fibroblasts by IL-1β treatment. Gene expression of IKBa, TNFAIP3 and TNIP1, which are negative-feedback regulators, were also increased after IL-1βtreatment in synovial fibroblasts. The increase in the expression of these genes was confirmed by real time-PCR analysis. The results suggest that IL-1β-responsive genes play important roles in the progression of inflammation and destruction of joint components.

Journal

• International Journal of Oral-Medical Sciences

  International Journal of Oral-Medical Sciences 12 (1), 5-12, 2013

  Research Institute of Oral Science, Nihon University School of Dentistry at Matsudo

Keywords

• synovial fibroblasts, temporomandibular joint, microarray analysis, signaling pathway analysis, interleukin-1β

Details

• CRID: 1390282680191179520
• NII Article ID: 130003375193
• DOI: 10.5466/ijoms.12.5
• ISSN: 21854254
• ISSN: 13479733
• Web Site: https://www.jstage.jst.go.jp/article/ijoms/12/1/12_5/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/2014375637
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed