High-Speed Autofocusing of Cells Using Radial Intensity Profiles Based on Depth From Diffraction (DFDi) Method

• Oku Hiromasa

  Graduate School of Information Science and Technology, University of Tokyo

• Makise Soshiro

  Aioi Insurance Co.,Ltd.

• Ishikawa Masatoshi

  Graduate School of Information Science and Technology, University of Tokyo

Abstract

In this paper we propose new image features named radial intensity profiles (RIP) for high-speed autofocusing of cells based on our Depth From Diffraction (DFDi) method. DFDi is a method of extracting depth information of target cells from a single defocused image containing their diffraction patterns. High-speed focusing of separated yeast cells with 20 ms response time and continuous autofocusing with a scanning microscope were demonstrated. The successful continuous autofocusing shows that image-based high-throughput measurement of cells could be realized using the proposed algorithm.

Journal

• Journal of Aero Aqua Bio-mechanisms

  Journal of Aero Aqua Bio-mechanisms 3 (1), 13-21, 2013

  Society of Aero Aqua Bio-mechanisms

Keywords

• Microscopy
• High Speed Autofocusing
• Cell
• Depth From Diffraction

Details

• CRID: 1390282680319607168
• NII Article ID: 130003375375
• DOI: 10.5226/jabmech.3.13
• ISSN: 21851522
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
  • KAKEN
• Abstract License Flag: Disallowed