Dynamic Changes in the Initial Colonization of <i>Actinomyces naeslundii</i> and <i>Streptococcus gordonii</i> Using a New Animal Model

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  • Zhang Xi
    Stomatological Hospital of Tianjin Medical University Department of Bacteriology I, National Institute of Infectious Diseases Department of Pediatric Dentistry, Nihon University Graduate School of Dentistry at Matsudo
  • Senpuku Hidenobu
    Department of Bacteriology I, National Institute of Infectious Diseases

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  • Dynamic Changes in the Initial Colonization of Actinomyces naeslundii and Streptococcus gordonii Using a New Animal Model

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Abstract

Actinomyces naeslundii and Streptococcus gordonii are the predominant bacteria and initial colonizers of oral microflora. The binding of A. naeslundii and S. gordonii and the interaction between them on the salivary pellicle-coated tooth surface play an important role in the biofilm development. Recently, we reported that NOD/SCID.e2f1− mice are a useful model for studying oral biofilm formation by Streptococcus mutans on the tooth surface. In this study, we aimed to determine whether NOD/SCID.e2f1− mice can be used for studying oral colonization of A. naeslundii and S. gordonii. Colonization of A. naeslundii in mice fed with 1% sucrose water for 24 h before inoculation was higher than that among mice fed with sucrose water for 1 h. A. naeslundii colonization using mixed species-inoculation was lower than that using single-species inoculation 30–90 min after inoculation; however, the colonization was higher 120–180 min after inoculation. The mixed inoculation induced better colonization of S. gordonii than single-species inoculation 60–180 min after inoculation. Polyclonal and fluorescein isothiocyanate-labeled antibody stained bacteria showed better colonization of S. gordonii when a mixed culture is used in vivo. NOD/SCID.e2f1− mice were useful for studying the initial colonization of A. naeslundii and S. gordonii. Long-term supply of sucrose water creates a favorable environment for the initial colonization of A. naeslundii that, in turn, supports the colonization of S. gordonii.

Journal

  • Japanese Journal of Infectious Diseases

    Japanese Journal of Infectious Diseases 66 (1), 11-16, 2013

    National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee

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