Multicolor Flow Cytometric Analyses of CD4<sup>+</sup> T Cell Responses to <i>Mycobacterium tuberculosis</i>-Related Latent Antigens
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- Yamashita Yoshiro
- Department of Clinical Medicine, Institute of Tropical Medicine, Nagasaki University
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- Hoshino Yoshihiko
- Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases
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- Oka Mayuko
- Division of Applied Life Science, Graduate School of Life and Environmental Sciences, Kyoto Prefectural University
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- Matsumoto Sokichi
- Department of Bacteriology, Osaka City University Graduate School of Medicine
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- Ariga Haruyuki
- Center for Respiratory Medicine, National Hospital Organization Tokyo National Hospital
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- Nagai Hideaki
- Center for Respiratory Medicine, National Hospital Organization Tokyo National Hospital
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- Makino Masahiko
- Department of Mycobacteriology, Leprosy Research Center, National Institute of Infectious Diseases
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- Ariyoshi Koya
- Department of Clinical Medicine, Institute of Tropical Medicine, Nagasaki University
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- Tsunetsugu-Yokota Yasuko
- Department of Immunology, National Institute of Infectious Diseases
Bibliographic Information
- Other Title
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- Multicolor Flow Cytometric Analyses of CD4+ T Cell Responses to Mycobacterium tuberculosis-Related Latent Antigens
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Abstract
Although IFN-γ release assays (IGRAs) provide increased specificity over tuberculin skin tests, the early and sensitive detection of reactivation of latently infected Mycobacterium tuberculosis is required to control tuberculosis (TB). Recently, a multicolor flow cytometry has been developed to study CD4+ T cell cytokine responses (IFN-γ/IL-2/TNF-α) to purified protein derivatives (PPD) and M. tuberculosis-specific antigens (ESAT-6/CFP-10) and provided useful information regarding anti-TB immunity. However, the diagnostic relevancy remains uncertain. Here, we analyzed three additional CD4+ T cell cytokine responses (IL-10/IL-13/IL-17) to latent mycobacterial antigens (α-crystallin, methylated heparin-binding hemagglutinin [HBHA], and mycobacterial DNA-binding protein 1 [MDP-1]) as well as PPD and ESAT-6/CFP-10 in 12 IGRA+ TB cases and 8 healthy controls. No significant difference in IFN-γ response was observed between TB cases and controls, which was likely due to the high variation among the individuals. However, we found a significant increase over healthy controls in (i) the IL-2 response to HBHA in recovery stage TB cases, (ii) the number of M. tuberculosis-specific polyfunctional CD4+ T cells in on-treatment and recovery stage cases, and (iii) the IL-17 response to HBHA and MDP-1 in on-treatment and recovery stage cases. These results suggest that a combination of these T cell cytokine parameters could aid in accurate diagnosis of latent TB infection.
Journal
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- Japanese Journal of Infectious Diseases
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Japanese Journal of Infectious Diseases 66 (3), 207-215, 2013
National Institute of Infectious Diseases, Japanese Journal of Infectious Diseases Editorial Committee
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Details 詳細情報について
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- CRID
- 1390001206239525504
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- NII Article ID
- 130003381673
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- NII Book ID
- AA1132885X
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- ISSN
- 18842836
- 13446304
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- HANDLE
- 10069/32996
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- NDL BIB ID
- 024523612
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- PubMed
- 23698481
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed