Evidence of Antagonistic Regulation of Restart from G₁ Delay in Response to Osmotic Stress by the Hog1 and Whi3 in Budding Yeast Evidence of Antagonistic Regulation of Restart from G<sub>1</sub> Delay in Response to Osmotic Stress by the Hog1 and Whi3 in Budding Yeast

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Author(s)

    • SHITAMUKAI Atsunori
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
    • TSUBAKIYAMA Ryohei
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
    • KOMARUYAMA Tadamasa
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
    • YAMAGUCHI Toshinaga
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
    • KUME Kazunori
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University
    • HIRATA Dai
    • Department of Molecular Biotechnology, Graduate School of Advanced Sciences of Matter, Hiroshima University

Abstract

Hog1 of <i>Saccharomyces cerevisiae</i> is activated by hyperosmotic stress, and this leads to cell-cycle delay in G<sub>1</sub>, but the mechanism by which cells restart from G<sub>1</sub> delay remains elusive. We found that Whi3, a negative regulator of G<sub>1</sub> cyclin, counteracted Hog1 in the restart from G<sub>1</sub> delay caused by osmotic stress. We have found that phosphorylation of Ser-568 in Whi3 by RAS/cAMP-dependent protein kinase (PKA) plays an inhibitory role in Whi3 function. In this study we found that the phosphomimetic Whi3 S568D mutant, like the Δ<i>whi3</i> strain, slightly suppressed G<sub>1</sub> delay of Δ<i>hog1</i> cells under osmotic stress conditions, whereas the non-phosphorylatable S568A mutation of Whi3 caused prolonged G<sub>1</sub> arrest of Δ<i>hog1</i> cells. These results indicate that Hog1 activity is required for restart from G<sub>1</sub> arrest under osmotic stress conditions, whereas Whi3 acts as a negative regulator for this restart mechanism.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 77(10), 2002-2007, 2013

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

Codes

  • NII Article ID (NAID)
    130003381877
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • ISSN
    0916-8451
  • NDL Article ID
    024971880
  • NDL Call No.
    Z53-G223
  • Data Source
    NDL  J-STAGE 
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