Steely Enzymes Are Involved in Prestalk and Prespore Pattern Formation

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Author(s)

Abstract

4-Methyl-5-pentylbenzene-1,3-diol (MPBD), a product of SteelyA enzyme, controls <i>Dictyostelium</i> spore maturation. Since the expression of <i>stlA</i> split the in early and terminal stages, we cannot exclude the possibility that MPBD regulates spore differentiation from the early stage by creating a bias between the cells. 1-(3,5-Dichloro-2,6-dihydroxy-4-methoxyphenyl) hexan-1-on (DIF-1), a product of SteelyB, was identified as the major stalk cell inducer by <i>in vitro</i> assay, but <i>in vivo</i> assay revealed that DIF-1 induces only prestalkB (pstB) and prestalkO (pstO) cells and, that the major prestalkA (pstA) cells differentiated without DIF-1. In order to determine mechanism of polyketide regulated pattern formation, we examined the spatial expression patterns of prestalk and prespore markers in <i>stlA</i> and <i>stlB</i> knockout mutants. We found that MPBD regulates spore maturation at the culmination stage. We also found that the <i>stlA</i> and <i>stlB</i> double-knockout mutant lost pstA marker gene expression.

Journal

  • Bioscience, Biotechnology, and Biochemistry

    Bioscience, Biotechnology, and Biochemistry 77(10), 2008-2012, 2013

    Japan Society for Bioscience, Biotechnology, and Agrochemistry

Codes

  • NII Article ID (NAID)
    130003381879
  • NII NACSIS-CAT ID (NCID)
    AA10824164
  • Text Lang
    ENG
  • ISSN
    0916-8451
  • NDL Article ID
    024971895
  • NDL Call No.
    Z53-G223
  • Data Source
    NDL  J-STAGE 
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